Sugita T, Nishikawa A, Shinoda T
Department of Microbiology, Meiji College of Pharmacy, Tokyo, Japan.
J Clin Microbiol. 1998 May;36(5):1458-60. doi: 10.1128/JCM.36.5.1458-1460.1998.
Trichosporon species are opportunistic pathogens, associated with a high mortality rate in immunocompromised patients. Oligonucleotide primers were used to amplify a 170-bp fragment of small-subunit ribosomal DNA of all species in the genus Trichosporon by PCR. The primers amplify DNAs of all species in the genus Trichosporon, including six causative agents of trichosporonosis. DNAs of other medically important yeasts, such as Candida albicans and Cryptococcus neoformans, are not amplified by this detection system.
毛孢子菌属是机会致病菌,在免疫功能低下的患者中具有较高的死亡率。使用寡核苷酸引物通过聚合酶链反应(PCR)扩增毛孢子菌属所有物种的小亚基核糖体DNA的170bp片段。这些引物可扩增毛孢子菌属所有物种的DNA,包括六种毛孢子菌病的病原体。其他医学上重要的酵母,如白色念珠菌和新型隐球菌的DNA,不会被该检测系统扩增。