Xiang Qiu, Tan Meng-qun, Huang Yan-hong
Laboratory of Blood Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Hunan Yi Ke Da Xue Xue Bao. 2002 Apr 28;27(2):108-10.
To explore the effect of sophora flavescens (SF) combined low molecular weight natural tumor suppressor(LMW-NTS) on normal human bone marrow CFU-GM and acute myelogenous leukemia cells and its mechanism.
CFU-GM colony culture, cell culture, Wrigh-Giemsa stain, 3H-TdR incorporation test, agarose gel electrophoresis of DNA fragment and RT-PCR were employed in this research.
The combination of SF and LMW-NTS greatly inhibited the proliferation of acute meylogenous leukemia cells, induced their apoptosis, and down-regulated the c-Myc expression as compared with SF or LMW-NTS alone.
The anti-leukemia effect of the combination of SF and LMW-NTS is superior to SF or LMW-NTS alone.
探讨苦参（SF）联合低分子量天然肿瘤抑制因子（LMW-NTS）对正常人骨髓CFU-GM及急性髓性白血病细胞的作用及其机制。
本研究采用CFU-GM集落培养、细胞培养、瑞氏-吉姆萨染色、3H-TdR掺入试验、DNA片段琼脂糖凝胶电泳及RT-PCR。
与单独使用SF或LMW-NTS相比，SF与LMW-NTS联合使用可显著抑制急性髓性白血病细胞的增殖，诱导其凋亡，并下调c-Myc表达。
SF与LMW-NTS联合使用的抗白血病效果优于单独使用SF或LMW-NTS。
