Chen Hua-Sheng, Xu Ai-Hua, Jia Lin-Chang, Ren Li
Medical College of Yangzhou University, Yangzhou 225001, China.
Zhongguo Zhong Yao Za Zhi. 2006 Nov;31(21):1822-4.
To study the effect of Shuangling Fuzheng anti-tumor preparation (SLAP) five groups on proliferation and c-myc gene expression of SGC-7901 cells in vitro.
The inhibitory effect of single SLAP (40 -640 microg x mL(-1)) and combined therapy with adriamycin (0.4, 4.0 microg x mL(-1) or cisplatin (0.1,1.0 microg x mL(-1) on human gastric carcinoma SGC-7901 cells proliferation were observed by MTT colorimetric analysis method. Technique of flow cytometry in vitro was used to measure the rate of positive sign of SLAP (80 - 320 microg x mL(-1)) on c-myc gene protein of SGC-7901 cells.
SGC-7901 cells proliferation were inhibited by single SLAP in dose of 40 - 640 microg x mL(-1) 24 h. Its inhibitory rate was increased with increase of dose. The inhibitory rate on SGC-7901 cells could be increased by SLAP in dose of 40 - 640 microg x mL(-1) plus adriamycin in dose of 0.4 and 4.0 microg x mL(-1) or plus cisplatin in dose of 0.1 and 1.0 microg x mL(-1). At the same time, SLAP (80 - 320 microg x mL(-1)) also could inhibite the expression of c-myc gene of SGC -7901 cells.
Single SLAP had inhibiting effect on human gastric carcinoma cells proliferation with a dose-effect relationship and synergic effect while combined with adriamycin or cisplatin. To inhibit the expression of c-myc gene of human gastric carcinoma cells might be one of action mechanisms of SLAP, which inhibited tumor cells proliferation.
研究双灵扶正抗肿瘤制剂（SLAP）五个剂量组对体外培养的SGC - 7901细胞增殖及c - myc基因表达的影响。
采用MTT比色分析法观察单剂量SLAP（40 - 640μg·mL⁻¹）及联合阿霉素（0.4、4.0μg·mL⁻¹）或顺铂（0.1、1.0μg·mL⁻¹）对人胃癌SGC - 7901细胞增殖的抑制作用。应用体外流式细胞术检测SLAP（80 - 320μg·mL⁻¹）对SGC - 7901细胞c - myc基因蛋白阳性率的影响。
单剂量40 - 640μg·mL⁻¹的SLAP作用24小时可抑制SGC - 7901细胞增殖，抑制率随剂量增加而升高。40 - 640μg·mL⁻¹的SLAP分别联合0.4和4.0μg·mL⁻¹的阿霉素或0.1和1.0μg·mL⁻¹的顺铂可增强对SGC - 7901细胞的抑制率。同时，80 - 320μg·mL⁻¹的SLAP也可抑制SGC - 7901细胞c - myc基因的表达。
单剂量SLAP对人胃癌细胞增殖有抑制作用，呈剂量 - 效应关系，与阿霉素或顺铂联合有协同作用。抑制人胃癌细胞c - myc基因表达可能是SLAP抑制肿瘤细胞增殖的作用机制之一。
