Ferguson Neil, Fersht Alan R
MRC Centre for Protein Engineering, MRC Centre, Hills Road, Cambridge CB2 2QH, UK.
Curr Opin Struct Biol. 2003 Feb;13(1):75-81. doi: 10.1016/s0959-440x(02)00009-x.
Recent advances have significantly increased the time and spectroscopic resolution of protein folding experiments. We can now study the timescale and nature of polypeptide collapse, and how this correlates with secondary and tertiary structure formation. Studies on ultrafast folding proteins and peptides provide experimental benchmarks on a timescale that overlaps directly with that of molecular dynamics simulations. This makes possible direct tests of both simulations and current models of protein folding.
最近的进展显著提高了蛋白质折叠实验的时间和光谱分辨率。现在我们能够研究多肽折叠的时间尺度和性质，以及这与二级和三级结构形成之间的关联。对超快折叠蛋白质和肽的研究在一个与分子动力学模拟直接重叠的时间尺度上提供了实验基准。这使得对模拟和当前蛋白质折叠模型进行直接测试成为可能。
