Hu Fangdi, Zhao Jianxiong, Feng Shilan, Liu Xin, Xu Jingwen
Lanzhou Medical College, Lanzhou.
Zhong Yao Cai. 2004 Nov;27(11):831-4.
To study on chromatography fingerprint(CFP) of Huangqi herbs.
HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution. CFP was referenced to formononetin, calycosin and then the contents of astragaloside IV, formononetin and calycosin was determinted.
26 common peaks were pointed out, their similarity of 12 samples were more than 90%.
The handling of samples were simple. The study method on chromatography fingerprint of Huangqi were reliable. Good repetition and their similarity accorded with the demand on "the technology guide to experiment studies on chromatography fingerprint of injection of traditional Chinese medicine (TCM)", which was issued by the nation pharmacopoeial commission. The chromatography fingerprint and determination of index composition could be used to comprehensively control the quality of Huangqi.
研究黄芪药材的色谱指纹图谱。
采用高效液相色谱法，以Hypersil ODS柱，水 - 乙腈梯度洗脱。以芒柄花素、毛蕊异黄酮为参照，测定黄芪甲苷、芒柄花素和毛蕊异黄酮的含量。
共指认26个共有峰，12个样品的相似度均大于90%。
样品处理方法简便，所建立的黄芪色谱指纹图谱研究方法可靠，重复性好，相似度符合国家药典委员会发布的《中药注射剂指纹图谱研究技术指南》要求。该色谱指纹图谱及指标成分测定可用于全面控制黄芪质量。
