Tanaka K, Fromm D
Surgery. 1983 May;93(5):660-3.
Little is known about the response of surface cells and glandular cells of the gastric mucosa to various injurious agents. This study examines the effects of bile acid and salicylate on oxygen consumption and membraneolysis of isolated gastric mucosal surface cells and compares these responses to those of cells constituting the gastric glands. The effects of salicylate and bile acid on oxygen consumption by surface cells are similar to previous observations for intact gastric glands and isolated mitochondria, indicating alterations of oxidative phosphorylation. Salicylate does not appreciably alter lipid release from either surface or glandular cells. However, bile acid causes a profound increase in lipid release from both surface and glandular cells. Salicylate does not alter the number of visibly intact surface or glandular cells, but 1 mM of taurocholic acid decreases the number of surface cells by 45% without altering the glandular cells. In contrast, 5 mM of taurocholic acid completely destroys the surface cells and reduces the number of gastric glandular cells by 51%. These data suggest that surface cells are more susceptible to membraneolytic effects of bile acid than are gastric glandular cells. This may account for the superficial nature of bile acid-induced injury to the gastric mucosa. Although both bile acid and salicylate interfere with oxidative metabolism, this effect alone does not appear to account for cellular destruction.
关于胃黏膜表面细胞和腺细胞对各种损伤因子的反应，人们了解甚少。本研究考察了胆汁酸和水杨酸盐对分离的胃黏膜表面细胞耗氧量和膜溶解的影响，并将这些反应与构成胃腺的细胞的反应进行比较。水杨酸盐和胆汁酸对表面细胞耗氧量的影响与之前对完整胃腺和分离线粒体的观察结果相似，表明氧化磷酸化发生了改变。水杨酸盐不会显著改变表面细胞或腺细胞的脂质释放。然而，胆汁酸会导致表面细胞和腺细胞的脂质释放大幅增加。水杨酸盐不会改变明显完整的表面细胞或腺细胞的数量，但1 mM牛磺胆酸会使表面细胞数量减少45%，而不会改变腺细胞数量。相比之下，5 mM牛磺胆酸会完全破坏表面细胞，并使胃腺细胞数量减少51%。这些数据表明，表面细胞比胃腺细胞更容易受到胆汁酸的膜溶解作用影响。这可能解释了胆汁酸诱导的胃黏膜损伤的表层性质。虽然胆汁酸和水杨酸盐都干扰氧化代谢，但仅这种作用似乎并不能解释细胞破坏的原因。
