Goel H C, Prem Kumar I, Rana S V S
Department of Radiation Biology, Institute of Nuclear Medicine and Allied sciences, Brig SK Majumdar Marg, Delhi 110 054.
Indian J Exp Biol. 2002 Jun;40(6):727-34.
Aqueous extract of T. cordifolia inhibited Fenton (FeSO4) reaction and radiation mediated 2-deoxyribose degradation in a dose dependent fashion with an IC50 value of 700 microg/ml for both Fenton and radiation mediated 2-DR degradation. Similarly, it showed a moderate but dose dependent inhibition of chemically generated superoxide anion at 500 microg/ml concentration and above with an IC50 value of 2000 microg/ml. Aqueous extract inhibited the formation of Fe2+-bipiridyl complex and formation of comet tail by chelating Fe2+ ions in a dose dependent manner with an IC50 value of 150 microg/ml for Fe2+-bipirydyl formation and maximally 200 microg/ml for comet tail formation, respectively. The extract inhibited ferrous sulphate mediated lipid peroxidation in a dose-dependent manner with an IC50 value of 1300 microg/ml and maximally (70%) at 2000 microg/ml. The results reveal that the direct and indirect antioxidant actions of T. cordifolia probably act in corroboration to manifest the overall radioprotective effects.
匙叶甘松水提取物以剂量依赖性方式抑制芬顿（硫酸亚铁）反应和辐射介导的2-脱氧核糖降解，对芬顿反应和辐射介导的2-DR降解的IC50值均为700微克/毫升。同样，在500微克/毫升及以上浓度时，它对化学产生的超氧阴离子表现出中等程度但剂量依赖性的抑制作用，IC50值为2000微克/毫升。水提取物通过螯合Fe2+离子以剂量依赖性方式抑制Fe2+-联吡啶配合物的形成和彗尾的形成，对Fe2+-联吡啶形成的IC50值为150微克/毫升，对彗尾形成的最大IC50值为200微克/毫升。该提取物以剂量依赖性方式抑制硫酸亚铁介导的脂质过氧化，IC50值为1300微克/毫升，在2000微克/毫升时最大抑制率为（70%）。结果表明，匙叶甘松的直接和间接抗氧化作用可能协同发挥作用，以体现其整体辐射防护效果。
