Kumar I P, Goel H C
Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi 110 054, India.
Indian J Exp Biol. 2000 Oct;38(10):1003-6.
Aqueous extract of Podophyllum species has been reported to render significant protection against radiation induced mortality, cytogenetic damage and cell death. In view of this, present study was undertaken to investigate its antioxidant properties. Chelation, oxidation and reduction of Fe2+ and Fe3+ were measured using chelating agents 2-2' bipiridyl and potassium thiocyanate respectively. Podophyllum extract, in a dose dependent manner, chelated Fe2+ more efficiently than Fe3+ and also modulated Fe2+/Fe3+ ratio. Homogenate of mouse liver was used to measure TBARS for estimating lipid peroxidation. Podophyllum extract also inhibited lipid peroxidation in a dose dependent manner and maximum inhibition (92%) was achieved at 1000 micrograms/ml concentration. These results demonstrates that Podophyllum exhibits antioxidant properties as seen through chelation and modulation of redox state of iron ions and these may primarily contribute towards its radioprotective manifestation.
据报道，鬼臼属植物的水提取物对辐射诱导的死亡率、细胞遗传损伤和细胞死亡具有显著的保护作用。鉴于此，本研究旨在探讨其抗氧化特性。分别使用螯合剂2,2'-联吡啶和硫氰酸钾测量Fe2+和Fe3+的螯合、氧化和还原情况。鬼臼提取物以剂量依赖的方式比Fe3+更有效地螯合Fe2+，并调节Fe2+/Fe3+比值。用小鼠肝脏匀浆测量硫代巴比妥酸反应物（TBARS）以评估脂质过氧化。鬼臼提取物也以剂量依赖的方式抑制脂质过氧化，在1000微克/毫升浓度下达到最大抑制率（92%）。这些结果表明，鬼臼通过螯合和调节铁离子的氧化还原状态表现出抗氧化特性，这些特性可能主要有助于其辐射防护表现。
