Mirejovská E, Mirejovský P, Hurych J
Physiol Bohemoslov. 1975;24(4):345-52.
Rats with subcutaneously implanted polyurethane sponges were exposed 6 hours daily for 7 days to high ambient atmospheric pressures (1.5, 2, 2.5 and 3 ATA). Another group was exposed 4 hours daily for 4 weeks to 3 ATA before inducing granulation tissue formation. 14C-proline was administered 16 hours before terminating the experiment. Free hydroxyproline, soluble and insoluble collagen and total noncollagenous protein were isolated from the 7-day granuloma and the amount and radioactivity of 14C-hydroxyproline and 14C-proline were determined. Seven days' graduated hyperbarism did not affect collagen synthesis; the maturation of collagen to insoluble forms was inhibited at 2 and 2.5 ATA, but not at 3 ATA. Stimulated degradation of collagen (free hydroxyproline) was observed at 2, 2.5 and 3 ATA. In animals subjected to long-term exposure at 3 ATA pressure, the collagen in the granuloma matured to insoluble forms more quickly. Biochemical changes were correlated with changes in the fine structure of the granulation tissue. The appearance of the fibroblast proteosynthetic apparatus was not influenced by hyperbarism. Progressive spherical transformation, fusion of mitochondria and lysosomal activation in the pericapillary fibroblasts occurred at 2, 2.5 and 3 ATA. In short-term experiment, the formation of cytosegresomes and cellular necrosis also contributed to the effect at 3 ATA, which is thus already a toxic pressure for granulation tissue.
皮下植入聚氨酯海绵的大鼠，每天暴露于高环境大气压力（1.5、2、2.5和3个绝对大气压）下6小时，持续7天。另一组在诱导肉芽组织形成前，每天暴露于3个绝对大气压下4小时，持续4周。在实验结束前16小时给予14C-脯氨酸。从7天的肉芽肿中分离出游离羟脯氨酸、可溶性和不溶性胶原蛋白以及总非胶原蛋白，并测定14C-羟脯氨酸和14C-脯氨酸的含量和放射性。7天的分级高压氧治疗不影响胶原蛋白合成；在2和2.5个绝对大气压下，胶原蛋白成熟为不溶性形式受到抑制，但在3个绝对大气压下不受影响。在2、2.5和3个绝对大气压下观察到胶原蛋白（游离羟脯氨酸）的刺激降解。在3个绝对大气压下长期暴露的动物中，肉芽肿中的胶原蛋白更快成熟为不溶性形式。生化变化与肉芽组织的精细结构变化相关。高压氧治疗不影响成纤维细胞蛋白质合成装置的外观。在2、2.5和3个绝对大气压下，毛细血管周围成纤维细胞出现进行性球形转化、线粒体融合和溶酶体激活。在短期实验中，3个绝对大气压下细胞分离体的形成和细胞坏死也产生了影响，因此3个绝对大气压对肉芽组织来说已经是一个有毒压力。
