Yoshimura K, Soulsby E J
Am J Trop Med Hyg. 1976 Jan;25(1):99-107. doi: 10.4269/ajtmh.1976.25.99.
Angiostrongylus cantonensis-infected rats were examined for the presence of antigen sensitive lymphocytes, as assessed by the in vitro uptake of tritiated thymidine by cells of various lymphoid organs (cervical, mediastinal and mesenteric lymph nodes, spleen and periphereal blood), following stimulation by adult worm antigen. The lymphoid cell response of rats to A. cantonensis appeared to be local in nature in that significant responses were noted only in the cervical lymph node cells during the first 4 weeks of infection. The responses of spleen cells to phytohemagglutinin gradually declined as the infection progressed and this reduced responsiveness was statistically significant during the period of 5 to 10 weeks of infection. Homocytotropic antibody, demonstrated by 72-hour homologous passive cutaneous anaphylaxis, was detected throughout 2 to 17 weeks postinfection with a peak response at the 5th week of infection. The antibody was heat labile and sensitive to reduction by 2-mercaptoethanol and alkylation. Hemagglutinating antibody was first observed 5 weeks after infection and high titers occurred throughout 6 to 17 weeks postinfection.
对广州管圆线虫感染的大鼠进行检测，以确定是否存在抗原敏感淋巴细胞。通过用成虫抗原刺激后，观察各种淋巴器官（颈部、纵隔和肠系膜淋巴结、脾脏和外周血）细胞对氚标记胸腺嘧啶核苷的体外摄取来评估。大鼠对广州管圆线虫的淋巴细胞反应似乎具有局部性，因为在感染的前4周，仅在颈部淋巴结细胞中观察到显著反应。随着感染的进展，脾细胞对植物血凝素的反应逐渐下降，在感染的5至10周期间，这种反应性降低具有统计学意义。通过72小时同源被动皮肤过敏反应证明的亲同种细胞抗体，在感染后2至17周内均可检测到，在感染第5周时反应达到峰值。该抗体对热不稳定，对2-巯基乙醇还原和烷基化敏感。血凝抗体在感染后5周首次观察到，在感染后6至17周期间出现高滴度。
