免疫抑制药物对淋巴结通透因子（LNPF）活性的影响。

Influence of immunosuppressive drugs on activity of lymph node permeability factor (LNPF).

作者信息

Kowalczyk-Bronisz S H, Gieldanowski J

出版信息

Arch Immunol Ther Exp (Warsz). 1976;24(1):143-7.

Abstract

The influence of selected immunosuppressive drugs on development of local inflammation induced by injection of LNPF in rats was studied. The factor was obtained from guinea pig lymph nodes stimulated with Freund's complete adjuvant. The cell-free extract was obtained by disintegrating the cells, dialysis and lyophilization of the material. Preventive action of the drugs was dose-related. Strongest protection against the effect of LNPF was observed after administration of 6-MP and cyclophosphamide (100 mg/kh). Methotrexate (20 mg/kg) caused a reduction of the surface area of the inflammatory reaction by 70-90%, and 5-fluorouracil (50 mg/kg) by 35%-60%. Specific heterologous anti-LNPF serum was the most effective protective agent in rats; administered before the test, it prevented appearance of an inflammatory reaction altogether.

摘要

研究了选用的免疫抑制药物对大鼠注射LNPF诱导的局部炎症发展的影响。该因子取自用弗氏完全佐剂刺激的豚鼠淋巴结。通过细胞裂解、透析和材料冻干获得无细胞提取物。药物的预防作用与剂量相关。给予6-巯基嘌呤和环磷酰胺（100mg/kg）后，观察到对LNPF作用的最强保护。甲氨蝶呤（20mg/kg）使炎症反应表面积减少70%-90%，5-氟尿嘧啶（50mg/kg）使炎症反应表面积减少35%-60%。特异性异源抗LNPF血清是大鼠中最有效的保护剂；在试验前给药，可完全防止炎症反应的出现。