Lisowska E, Duk M
Arch Immunol Ther Exp (Warsz). 1976;24(1):39-45.
M and N blood group glycoproteins from O group erythrocytes were hydrolyzed with acid under various conditions that produced a gradual release of sialic acid. The preparations desialized to different degree were tested for M, N, NVg and AHp activities. M and N blood group activity was decreased upon release of sialic acid, and AHp activity was exposed "de novo" and increased upon desialization. NVg activity always increased during the initial period of acid hydrolysis, but prolonged heating in acid solution caused subsequent partial inactivation of glycoprotein. This decrease in NHp activity was not related either to the extent of desialization, or to fragmentation of the glycoprotein molecule.
来自O型红细胞的M和N血型糖蛋白在各种条件下用酸水解，这些条件会使唾液酸逐渐释放。对脱唾液酸程度不同的制剂进行M、N、NVg和AHp活性测试。唾液酸释放后，M和N血型活性降低，脱唾液酸时“从头”暴露并增加AHp活性。在酸水解初期，NVg活性总是增加，但在酸性溶液中长时间加热会导致糖蛋白随后部分失活。NHp活性的这种降低既与脱唾液酸程度无关，也与糖蛋白分子的断裂无关。
