Lisowska E, Kordowicz M
Vox Sang. 1977 Sep;33(3):164-9. doi: 10.1111/j.1423-0410.1977.tb02248.x.
Antisera obtained by immunizing rabbits with desialized M and N blood group glycoproteins (M' and N' glycoproteins) agglutinate desialized erythrocytes and precipitate desialized glycoproteins, but they do not react with sialic acid containing erythrocytes or glycoproteins. By absorption of anti-M' serum with N' glycoprotein, and of anti-N' serum with M' glycoprotein, reagents specifically agglutinating M' or N' erythrocytes were obtained. The absorbed anti-M' and anti-N' sera were specifically inhibited by M' or N' glycoproteins, respectively. The results show that sialic acid residues of red cell glycoproteins are not essential for the differentiation of M an N blood group determinants.
用脱唾液酸的M和N血型糖蛋白（M'和N'糖蛋白）免疫兔子获得的抗血清，能使脱唾液酸的红细胞发生凝集，并使脱唾液酸的糖蛋白沉淀，但它们不与含唾液酸的红细胞或糖蛋白发生反应。通过用N'糖蛋白吸收抗M'血清，并用M'糖蛋白吸收抗N'血清，获得了特异性凝集M'或N'红细胞的试剂。吸收后的抗M'和抗N'血清分别被M'或N'糖蛋白特异性抑制。结果表明，红细胞糖蛋白的唾液酸残基对于M和N血型决定簇的区分并非必不可少。
