利用随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）研究产抗生素土壤链霉菌的基因型和表型特征。

Genotypic and phenotypic characteristics of antibiotic-producing soil Streptomyces investigated by RAPD-PCR.

作者信息

Gharaibeh Raad, Saadoun Ismail, Mahasneh Amjad

机构信息

Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid-22110, Jordan.

出版信息

J Basic Microbiol. 2003;43(1):18-27. doi: 10.1002/jobm.200390000.

DOI:10.1002/jobm.200390000

PMID:12596238

Abstract

Random amplified polymorphic DNA (RAPD) analysis has been used to determine the relatedness of 73 antibiotic-producing soil Streptomyces isolates that were recovered from different soil habitats in Jordan based on their RAPD-PCR fingerprints. Genetic polymorphisms between these isolates showed three common bands of 2777, 800 and 250 bp shared by approximately (95%) of them. Some specific bands were also observed. Further analysis of RAPD patterns with the UPGMA resulted in clustering the tested isolates into two main super clusters. Super cluster I was more homogenous than super cluster II and contained all the reference strains. However, super cluster II consists of unrelated isolates within five small groups. As RAPD fingerprints of the tested isolates linked to their phenotypes, differentiation between isolates with different cultural properties was observed.

摘要

随机扩增多态性DNA（RAPD）分析已被用于确定从约旦不同土壤生境中分离出的73株产抗生素土壤链霉菌菌株之间的亲缘关系，该分析基于它们的RAPD-PCR指纹图谱。这些菌株之间的遗传多态性显示出三条常见条带，大小分别为2777 bp、800 bp和250 bp，约95%的菌株都有这三条条带。还观察到一些特异性条带。用UPGMA对RAPD图谱进行进一步分析，结果将受试菌株聚为两个主要的超级聚类。超级聚类I比超级聚类II更具同质性，并且包含所有参考菌株。然而，超级聚类II由五个小群体中不相关的菌株组成。由于受试菌株的RAPD指纹图谱与其表型相关，因此观察到具有不同培养特性的菌株之间存在差异。