通过随机扩增多态性DNA聚合酶链反应（RAPD-PCR）对温泉源中分离出的耐热芽孢杆菌菌株进行遗传多态性及表型特征分析。

Genetic polymorphism by RAPD-PCR and phenotypic characteristics of isolated thermotolerant Bacillus strains from hot spring sources.

作者信息

Hazem A, Manar A

机构信息

Hashemite University, Faculty of Allied Health Sciences, Department of Medical Laboratory Sciences, Zarqa, Jordan.

出版信息

New Microbiol. 2003 Jul;26(3):249-56.

PMID:12901420

Abstract

The polymerase chain reaction (PCR) based random amplified polymorphic DNA (RAPD) assay, morphological, physiological, biochemical and antimicrobial susceptibility test methods have been evaluated for use in the taxonomy of isolated thermotolerant Bacillus from Jordanian hot springs, with specific reference to strains Geobacillus stearothermophilus (ATCC 12980), Bacillus circulans (ATCC 4513) and Bacillus sphaericus (ATCC 14577). A RAPD assay has been optimized and is able to discriminate between numerous thermotolerant Bacillus strains. RAPD-PCR was found to give reproducible thermotolerant Bacillus strains classification of DNA fingerprints for 14 strains including 3 reference strains. A study of 14 isolates and 3 reference strains, analyzing 53 phenotypic characters, resulted in their allocation to five major clusters at 60% similarity. Whereas at 80% similarity, twelve taxonomically distinct groups were evident.

摘要

基于聚合酶链反应（PCR）的随机扩增多态性DNA（RAPD）分析、形态学、生理学、生化及抗菌药敏试验方法已用于对从约旦温泉中分离出的耐热芽孢杆菌进行分类研究，特别参考嗜热栖热放线菌（ATCC 12980）、环状芽孢杆菌（ATCC 4513）和球形芽孢杆菌（ATCC 14577）菌株。已优化RAPD分析，其能够区分众多耐热芽孢杆菌菌株。发现RAPD-PCR可为包括3株参考菌株在内的14株耐热芽孢杆菌菌株生成可重复分类的DNA指纹图谱。对14株分离株和3株参考菌株进行研究，分析53个表型特征，结果在60%的相似性水平下将它们分为五个主要类群。而在80%的相似性水平下，有12个分类上不同的组明显可见。