Obara Naomi, Hasegawa Morifumi, Kodama Osamu
United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-city, Tokyo 183-8509, Japan.
Biosci Biotechnol Biochem. 2002 Dec;66(12):2549-59. doi: 10.1271/bbb.66.2549.
The volatiles released from elicitor (copper chloride, jasmonic acid, UV, L-methionine and chitosan oligomer)-treated and rice blast fungus-inoculated rice leaves were collected by the solid-phase microextraction technique and analyzed by GC-MS. (Z)-3-Hexen-1-ol, monoterpenes, methyl salicylate, and sesquiterpenes were identified as elicitor-induced volatiles by a comparison of their GC retention times and mass spectra with those of authentic compounds. The different elicitors resulted in some qualitative and quantitative differences in the production of volatiles. Monoterpenes and sesquiterpenes were identified as the rice blast fungus-induced volatiles.
采用固相微萃取技术收集经激发子(氯化铜、茉莉酸、紫外线、L-甲硫氨酸和壳寡糖)处理并接种稻瘟病菌的水稻叶片释放的挥发性物质,并用气相色谱-质谱联用仪进行分析。通过将其气相色谱保留时间和质谱与标准化合物的进行比较,鉴定出(Z)-3-己烯-1-醇、单萜类、水杨酸甲酯和倍半萜类为激发子诱导的挥发性物质。不同的激发子在挥发性物质的产生上导致了一些定性和定量的差异。单萜类和倍半萜类被鉴定为稻瘟病菌诱导的挥发性物质。