裙带菜提取物与壳聚糖联合应用协同激活豌豆对白粉病的宿主防御。

Combined application of Ascophyllum nodosum extract and chitosan synergistically activates host-defense of peas against powdery mildew.

机构信息

Department of Plant Food and Environmental Sciences, Dalhousie University, Nova Scotia, Canada.

出版信息

BMC Plant Biol. 2020 Mar 12;20(1):113. doi: 10.1186/s12870-020-2287-8.

BACKGROUND

Powdery mildew (PM) is an important disease of pea that reduce yield. Ascophyllum nodosum extract (ANE) and chitosan (CHT) are biostimulants used to improve plant health. Efficacy of ANE and CHT was assessed individually and in combination against pea powdery mildew.

RESULTS

Combined applications of ANE and CHT had a significant inhibitory effect on pathogen development and it reduced disease severity to 35%, as compared to control (90.5%). The combination of ANE and CHT enhanced the activity of plant defense enzymes; phenylalanine ammonia lyases (PAL), peroxidase (PO) and production of reactive oxygen species (ROS) and hydrogen peroxide (HO). Further, the treatment increased the expression of a number of plant defense genes in jasmonic acid (JA) signaling pathway such as LOX1 and COI and salicylic acid (SA)-mediated signaling pathway such as NPR1 and PR1. Other genes involved in defense mechanisms like NADPH oxidase and C4H were also upregulated by the combination treatment.

CONCLUSION

The combination of ANE and CHT suppresses pea powdery mildew largely by modulating JA and SA-mediated signaling pathways.

背景

白粉病是豌豆的一种重要病害，会降低产量。裙带菜提取物（ANE）和壳聚糖（CHT）是两种用于改善植物健康的生物刺激素。分别评估了 ANE 和 CHT 的功效及其组合对豌豆白粉病的防治效果。

结果

ANE 和 CHT 的联合应用对病原菌的发展有显著的抑制作用，与对照（90.5%）相比，病情严重度降低到 35%。ANE 和 CHT 的组合增强了植物防御酶的活性；苯丙氨酸解氨酶（PAL）、过氧化物酶（PO）和活性氧（ROS）和过氧化氢（HO）的产生。此外，该处理增加了茉莉酸（JA）信号通路中许多植物防御基因的表达，如 LOX1 和 COI，以及水杨酸（SA）介导的信号通路中如 NPR1 和 PR1 的表达。组合处理还上调了其他参与防御机制的基因，如 NADPH 氧化酶和 C4H。