利用随机扩增多态性DNA聚合酶链式反应（RAPD-PCR）分析不同鹅品系的DNA多态性。

DNA polymorphism in various goose lines by RAPD-PCR.

作者信息

Bednarczyk Marek, Siwek Maria, Mazanowski Adam, Czekalski Przemysław

机构信息

National Research Institute of Animal Production, Poultry Research Division, Zakrzewo nr. Poznań, Poznańska 11, 62-069 Palledzie, Poland.

出版信息

Folia Biol (Krakow). 2002;50(1-2):45-8.

PMID:12597533

Abstract

RAPD markers often primers were used to assess the polymorphism among pooled DNA of eight goose lines. The number of bands amplified by each primer ranged from 1 to 8, within a mean of 2.86. Some bands appeared specific for the line or genetic background. RAPD technique is an effective method for generating the polymorphic DNA marker in the goose. RAPD patterns from mixed DNA samples can reflect the genetic information of populations. The present study indicated that 10 generations selected for egg production and body weight under various pressure, resulted in genetic variation among goose lines as detected by RAPD. Selection for meat traits caused greater genetic diversity than selection for egg production.

摘要

随机扩增多态性DNA（RAPD）标记常被用于评估八个鹅品系混合DNA之间的多态性。每个引物扩增出的条带数在1至8条之间，平均为2.86条。有些条带对特定品系或遗传背景具有特异性。RAPD技术是在鹅中产生多态性DNA标记的有效方法。混合DNA样本的RAPD图谱能够反映群体的遗传信息。本研究表明，在不同压力下对产蛋量和体重进行10代选育后，通过RAPD检测发现鹅品系间产生了遗传变异。对肉用性状的选育比产蛋性状的选育导致了更大的遗传多样性。