鹅的雌性特异性DNA序列

Female-specific DNA sequences in geese.

Huang M C, Lin W C, Horng Y M, Rouvier R, Huang C W

Department of Animal Science, National Chung Hsing University, Taichung, Taiwan.

Br Poult Sci. 2003 Jul;44(3):359-64. doi: 10.1080/0007166031000085562.

Abstract

The OPAE random primers (Operon Technologies, Inc., CA) were used for random amplified polymorphic DNA (RAPD) fingerprinting in Chinese, White Roman and Landaise geese. One of these primers, OPAE-06, produced a 938-bp sex-specific fragment in all females and in no males of Chinese geese only. 2. A novel female-specific DNA sequence in Chinese goose was cloned and sequenced. Two primers, CGSex-F and CGSex-R, were designed in order to amplify a 912-bp sex-specific polymerase chain reaction (PCR) fragment on genomic DNA from female geese. 3. It was shown that a simple and effective PCR-based sexing technique could be used in the three goose breeds studied. 4. Nucleotide sequencing of the sex-specific fragments in White Roman and Landaise geese was performed and sequence differences were observed among these three breeds.

摘要

使用OPAE随机引物（Operon Technologies公司，加利福尼亚州）对中国鹅、白罗曼鹅和朗德鹅进行随机扩增多态性DNA（RAPD）指纹分析。其中一个引物OPAE - 06仅在中国鹅的所有雌性个体中产生了一个938 bp的性别特异性片段，雄性个体中未出现。2. 克隆并测序了中国鹅中一个新的雌性特异性DNA序列。设计了两个引物CGSex - F和CGSex - R，用于从雌性鹅的基因组DNA中扩增出一个912 bp的性别特异性聚合酶链反应（PCR）片段。3. 结果表明，一种简单有效的基于PCR的性别鉴定技术可用于所研究的三个鹅品种。4. 对白罗曼鹅和朗德鹅的性别特异性片段进行了核苷酸测序，并观察到这三个品种之间的序列差异。