Woo Newton H, Nguyen Peter V
Department of Physiology, University of Alberta School of Medicine, Edmonton, Alberta, T6G 2H7, Canada.
J Neurosci. 2003 Feb 15;23(4):1125-32. doi: 10.1523/JNEUROSCI.23-04-01125.2003.
De novo protein synthesis and transcription are necessary for the expression of long-lasting synaptic potentiation [long-term potentiation (LTP)] in hippocampal area CA1 and for the consolidation of long-term memory. The stability of LTP and its longevity require macromolecular synthesis at later stages, but a specific role for early protein synthesis has not been identified. Using electrophysiological recording methods in mouse hippocampal slices, we show that multiple trains of high-frequency stimulation provide immediate synaptic immunity to depotentiation. This immunity to depotentiation is dependent on the amount of synaptic stimulation used to induce LTP, it is input specific, and it is prevented by inhibitors of protein synthesis. We propose that local translation mediates input-specific synaptic immunity against depotentiation. We also present evidence suggesting that, in addition to translation, products of transcription can provide cell-wide immunity to depotentiation via heterosynaptic transfer of synaptic immunity between distinct pathways in area CA1. Protein synthesis and transcription may importantly regulate long-term storage of information by conferring synaptic immunity to depotentiation at previously potentiated synapses.
从头合成蛋白质和转录对于海马体CA1区持久的突触增强[长期增强(LTP)]的表达以及长期记忆的巩固是必要的。LTP的稳定性及其持久性在后期需要大分子合成，但早期蛋白质合成的具体作用尚未明确。通过在小鼠海马体切片中使用电生理记录方法，我们发现多串高频刺激可立即为去增强提供突触免疫。这种对去增强的免疫取决于用于诱导LTP的突触刺激量，具有输入特异性，并且会被蛋白质合成抑制剂阻断。我们提出局部翻译介导针对去增强的输入特异性突触免疫。我们还提供了证据表明，除了翻译之外，转录产物可以通过CA1区不同通路之间突触免疫的异突触转移为去增强提供全细胞免疫。蛋白质合成和转录可能通过赋予先前增强的突触对去增强的突触免疫来重要地调节信息的长期存储。
