Huang Yan-You, Kandel Eric R
Center for Neurobiology and Behavior, New York State Psychiatric Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
Learn Mem. 2005 Nov-Dec;12(6):587-93. doi: 10.1101/lm.98905. Epub 2005 Nov 14.
The late phase of LTP (L-LTP) is typically induced by repeated high-frequency stimulation. This form of LTP requires activation of transcription and translation and results in the cell-wide distribution of gene products that can be captured by other marked synapses. Here we report that theta frequency stimulation (5 Hz, 30 sec) applied to the Schaeffer-collateral pathway can induce a form of late phase of LTP that is restricted locally to the dendritic compartment. The late phase of theta frequency LTP is maintained even in isolated CA1 dendrites and is dependent on dendritic translation and actin cytoskeletal regulation, but is independent of transcription. This local form of L-LTP is not accessible to synaptic capture by other synapses, indicating that this form of LTP is restricted to the synaptic compartment. These results indicate that different patterns of synaptic stimulation can induce distinct forms of LTP that may have different roles in memory storage.
长时程增强效应的晚期阶段（L-LTP）通常由重复高频刺激诱导产生。这种形式的LTP需要转录和翻译的激活，并导致基因产物在整个细胞中分布，这些产物可被其他标记的突触捕获。在此我们报告，施加于谢弗侧支通路的θ频率刺激（5赫兹，30秒）可诱导一种晚期阶段的LTP，其在局部仅限于树突区域。即使在分离的CA1树突中，θ频率LTP的晚期阶段也能维持，并且依赖于树突翻译和肌动蛋白细胞骨架调节，但与转录无关。这种局部形式的L-LTP无法被其他突触进行突触捕获，这表明这种形式的LTP仅限于突触区域。这些结果表明，不同的突触刺激模式可诱导出不同形式的LTP，它们在记忆存储中可能具有不同的作用。
