Board Kathryn F, Patil Sangita, Lebedeva Irina, Capuano Saverio, Trichel Anita M, Murphey-Corb Michael, Rajakumar Premeela A, Flynn JoAnne L, Haidaris Constantine G, Norris Karen A
Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
J Infect Dis. 2003 Feb 15;187(4):576-88. doi: 10.1086/373997. Epub 2003 Feb 7.
To establish experimental Pneumocystis carinii infection in simian immunodeficiency virus (SIV)-infected macaques as a model of acquired immunodeficiency syndrome (AIDS)-associated P. carinii pneumonia (PCP), SIV-infected macaques were inoculated intrabronchially with macaque-derived P. carinii, and P. carinii-specific polymerase chain reaction (PCR) and flow cytometric analysis of bronchoalveolar lavage fluid were done biweekly for up to 44 weeks after inoculation. All inoculated animals had a P. carinii-specific PCR product after infection. CD8(+) T cells in lung lavage samples from SIV- and P. carinii-coinfected animals increased to >90% of total CD3(+) cells, a pattern associated with naturally acquired P. carinii infection. Progression of disease also was correlated with increased neutrophil infiltration to the lungs. The animals had a protracted period of asymptomatic colonization with P. carinii before progression to PCP. The development of a model of PCP in SIV-infected rhesus macaques provides the means to study AIDS-associated PCP.
为了在感染猿猴免疫缺陷病毒（SIV）的猕猴中建立实验性卡氏肺孢子虫感染，以此作为获得性免疫缺陷综合征（AIDS）相关的卡氏肺孢子虫肺炎（PCP）的模型，将猕猴来源的卡氏肺孢子虫经支气管接种到感染SIV的猕猴体内，并在接种后长达44周的时间里每两周进行一次卡氏肺孢子虫特异性聚合酶链反应（PCR）以及支气管肺泡灌洗 fluid 的流式细胞术分析。所有接种动物在感染后均有卡氏肺孢子虫特异性PCR产物。来自SIV和卡氏肺孢子虫共同感染动物的肺灌洗样本中的CD8(+) T细胞增加至总CD3(+)细胞的>90%，这一模式与自然获得的卡氏肺孢子虫感染相关。疾病进展也与肺部中性粒细胞浸润增加相关。在进展为PCP之前，这些动物有一段卡氏肺孢子虫无症状定植的漫长时期。在感染SIV的恒河猴中建立PCP模型为研究AIDS相关的PCP提供了手段。
