Babior Bernard M, Takeuchi Cindy, Ruedi Julie, Gutierrez Abel, Wentworth Paul
Department of Molecular and Experimental Medicine, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3031-4. doi: 10.1073/pnas.0530251100. Epub 2003 Feb 24.
Recent studies have suggested that antibodies can catalyze the generation of previously unknown oxidants including dihydrogen trioxide (H(2)O(3)) and ozone (O(3)) from singlet oxygen ((1)O(2)()) and water. Given that neutrophils have the potential both to produce (1)O(2)() and to bind antibodies, we considered that these cells could be a biological source of O(3). We report here further analytical evidence that antibody-coated neutrophils, after activation, produce an oxidant with the chemical signature of O(3). This process is independent of surface antibody concentration down to 50% of the resting concentration, suggesting that surface IgG is highly efficient at intercepting the neutrophil-generated (1)O(2)(*). Vinylbenzoic acid, an orthogonal probe for ozone detection, is oxidized by activated neutrophils to 4-carboxybenzaldehyde in a manner analogous to that obtained for its oxidation by ozone in solution. This discovery of the production of such a powerful oxidant in a biological context raises questions about not only the capacity of O(3) to kill invading microorganisms but also its role in amplification of the inflammatory response by signaling and gene activation.
最近的研究表明，抗体可以催化由单线态氧（¹O₂*）和水生成包括三氧化二氢（H₂O₃）和臭氧（O₃）在内的先前未知的氧化剂。鉴于中性粒细胞既有产生¹O₂的潜力，又能结合抗体，我们认为这些细胞可能是O₃的生物来源。我们在此报告进一步的分析证据，即抗体包被的中性粒细胞在激活后会产生具有O₃化学特征的氧化剂。该过程与表面抗体浓度无关，低至静息浓度的50%，这表明表面IgG在拦截中性粒细胞产生的¹O₂方面非常高效。乙烯基苯甲酸是一种用于臭氧检测的正交探针，被活化的中性粒细胞氧化为4 - 羧基苯甲醛，其方式类似于在溶液中被臭氧氧化的情况。在生物环境中发现这种强氧化剂的产生不仅引发了关于O₃杀死入侵微生物能力的问题，还引发了其在通过信号传导和基因激活放大炎症反应中作用的问题。
