Meyer Wieland, Castañeda Alexandra, Jackson Stuart, Huynh Matthew, Castañeda Elizabeth
University of Sydney, Sydney, Australia.
Emerg Infect Dis. 2003 Feb;9(2):189-95. doi: 10.3201/eid0902.020246.
A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphism analysis with HhaI and Sau96I in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gatii serotypes B and C isolates.
建立了一个网络，以获取伊比利亚美洲国家新型隐球菌的基础知识。为此，使用M13聚合酶链反应指纹图谱和乳清苷单磷酸焦磷酸化酶（URA5）基因限制性片段长度多态性分析，对来自阿根廷、巴西、智利、哥伦比亚、墨西哥、秘鲁、委内瑞拉、危地马拉和西班牙的340株临床、兽医和环境分离株进行了双酶切，用HhaI和Sau96I进行分析。两种技术都将所有分离株分为八个先前确定的分子类型。大多数分离株，即68.2%（n = 232），为VNI（格氏变种，血清型A），这与该变种在全球引起大多数人类隐球菌感染的事实相符。较小比例的分离株，即5.6%（n = 19），为VNII（格氏变种，血清型A）；4.1%（n = 14）为VNIII（AD杂交型），其中9株在URA5基因中有多态性；1.8%（n = 6）为VNIV（新型变种，血清型D）；3.5%（n = 12）为VGI；6.2%（n = 21）为VGII；9.1%（n = 31）为VGIII，1.5%（n = 5）为VGIV，所有四种VG类型均包含加蒂变种血清型B和C的分离株。
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