Shida Kazuhito, Terajima Daichi, Uchino Ryuji, Ikawa Shuntaro, Ikeda Makoto, Asano Katsutoshi, Watanabe Toshio, Azumi Kaoru, Nonaka Masaru, Satou Yutaka, Satoh Nori, Satake Masanobu, Kawazoe Yoshiyuki, Kasuya Atsuo
Center for Interdisciplinary Research, Tohoku University, Sendai, Japan.
Biochem Biophys Res Commun. 2003 Mar 7;302(2):207-18. doi: 10.1016/s0006-291x(03)00113-x.
Ascidians, which are classified as urochordata, appear to employ a primitive system of host defense that is considered to be a prototype of vertebrate innate immunity. We performed a cDNA/EST study to identify the genes expressed in the hemocytes of Ciona intestinalis. We obtained 3357 one-path reads that were then grouped into 1889 independent clusters. Although two thirds of the clusters could not be assigned to any particular gene, the remaining 530 clusters had significant homology to genes with known function. Of these, 62 clusters appeared to be related to host defense mechanisms. These include transcripts whose products are probably involved in cytotoxicity, detoxification, inflammation, and apoptosis. As expected, elements of acquired immunity were not detected. Thus, Ciona hemocytes appear to express a number of host defense-related genes involved in innate immune mechanisms.
被归类为尾索动物的海鞘，似乎采用了一种原始的宿主防御系统，该系统被认为是脊椎动物先天免疫的原型。我们进行了一项cDNA/EST研究，以鉴定在玻璃海鞘血细胞中表达的基因。我们获得了3357条单通路读数，然后将其分组为1889个独立的簇。尽管三分之二的簇无法归属于任何特定基因，但其余530个簇与已知功能的基因具有显著的同源性。其中，62个簇似乎与宿主防御机制有关。这些包括其产物可能参与细胞毒性、解毒、炎症和凋亡的转录本。正如预期的那样，未检测到获得性免疫的成分。因此，玻璃海鞘血细胞似乎表达了许多参与先天免疫机制的宿主防御相关基因。
