van Waarde D, Hulsing-Hesselink E, van Furth R
Cell Tissue Kinet. 1976 Jan;9(1):51-63.
An intraperitoneal injection of newborn calf serum (NBCS) into CRF Swiss mice causes an inflammatory reaction characterized by an increase in the number of macrophages in the peritoneal cavity and a concomitant monocytosis. The serum of such mice contains a monocytosis-inducing factor, as demonstrated by the intravenous injection of serum collected 18 (CalS18) and 24 hr (CalS24) after the intraperitoneal injection of NBCS. Serum from normal untreated mice, from mice given an intraperitoneal injection of sterile pyrogen-free saline, which does not cause an inflammatory reaction, or from mice 72 hr after an intraperitoneal injection of NBCS, when the inflammatory reaction has subsided, does not cause a monocytosis in test mice. Intravenous injection of CalS18 causes not only a monocytosis but also an increase in the number of promonocytes and bone marrow monocytes, suggesting an increased in the number of promonocytes and bone marrow monocytes, suggesting an increased production of monocytes. The effect of CalS18, CalS24 and CalS18 filtrate is specific for the mononuclear phagocytes, since only non-significant increases in the numbers of lymphocytes and granulocytes were observed. The active factor in CalS18 was shown to be different from the monocytosis-inducing factor present in NBCS. The monocytosis-inducing factor in CalS18 passes through an ultrafiltration membrane with an exclusion limit of 50,000 Daltons, so that the molecular weight must be below this value.
向慢性肾功能衰竭（CRF）的瑞士小鼠腹腔内注射新生小牛血清（NBCS）会引发炎症反应，其特征为腹腔内巨噬细胞数量增加以及伴随的单核细胞增多。静脉注射腹腔注射NBCS后18小时（CalS18）和24小时（CalS24）采集的血清表明，此类小鼠的血清中含有单核细胞增多诱导因子。正常未处理小鼠的血清、腹腔注射无菌无热原盐水（不会引发炎症反应）的小鼠的血清，或腹腔注射NBCS 72小时后（此时炎症反应已消退）的小鼠的血清，均不会在受试小鼠中引起单核细胞增多。静脉注射CalS18不仅会导致单核细胞增多，还会使前单核细胞和骨髓单核细胞数量增加，这表明单核细胞的产生有所增加。CalS18、CalS24和CalS18滤液的作用对单核吞噬细胞具有特异性，因为仅观察到淋巴细胞和粒细胞数量有不显著的增加。结果表明，CalS18中的活性因子与NBCS中存在的单核细胞增多诱导因子不同。CalS18中的单核细胞增多诱导因子可透过截留分子量为50,000道尔顿的超滤膜，因此其分子量必定低于此值。
