Eliopoulos G, André S, Halpern B
Dev Biol Stand. 1977;38:183-8.
Intravenous injection of 548 microgram of C. parvum (Lot 0407, Mérieux Laboratories, France) into C57Bl mice produced rapidly appearing monocytopenia which was followed by marked and prolonged monocytosis after the third day. The serum of these animals, collected during the monocytopenic but not the monocytosis phase, showed monocytosis inducing activity (MIA) as was demonstrated by the intravenous injection of the serum into normal test mice. Serum from normal untreated mice or from mice given an intravenous injection of sterile pyrogen-free saline did not cause monocytosis in the test mice. Monocytosis induced in the test animals presented two interesting peaks. The first was observed 2 h after the injection of serum and the second 5 days later. The former was accompanied by a decrease and the latter by an increase in the number of bone marrow monocytes, suggesting that MIA probably represents a releasing activity. The late increase in marrow monocytes is considered as a phenomenon secondary to the initial reduction.
给C57Bl小鼠静脉注射548微克微小隐孢子虫（批号0407，法国梅里埃实验室）后，小鼠迅速出现单核细胞减少，第三天后出现明显且持续时间较长的单核细胞增多。在单核细胞减少期而非单核细胞增多期采集的这些动物的血清，经静脉注射到正常试验小鼠体内后显示出单核细胞增多诱导活性（MIA）。来自未处理的正常小鼠或静脉注射无菌无热原生理盐水的小鼠的血清，在试验小鼠中未引起单核细胞增多。试验动物中诱导的单核细胞增多呈现出两个有趣的峰值。第一个峰值在注射血清后2小时观察到，第二个在5天后观察到。前者伴随着骨髓单核细胞数量的减少，后者伴随着骨髓单核细胞数量的增加，这表明MIA可能代表一种释放活性。骨髓单核细胞的后期增加被认为是初始减少后的继发现象。
