Sluiter W, Nibbering P H, Van Furth R, Annema A, Elzenga-Claasen I, Van Hemsbergen-Oomens L W, Van der Voort Van der Kleij-Van Andel A
Department of Infectious Diseases, University Hospital, Leiden, The Netherlands.
Immunology. 1990 Jul;70(3):327-31.
In mice given an intravenous injection of Mycobacterium bovis (BCG), the bacilli proliferated in the spleen, liver and lungs but the peritoneal cavity remained sterile. The numbers of blood monocytes and alveolar macrophages were increased during the first 2 weeks of the infection, whereas the number of peritoneal macrophages remained constant. To study whether factor-increasing monocytopoiesis (FIM) plays a role in the regulation of the monocytosis during the BCG infection, the activity of this factor in the serum of mice at various intervals during the infection was determined. Previous studies have shown that FIM stimulates monocyte production by its effect on the mitotic activity of monoblasts and promonocytes in the bone marrow. The FIM activity of the serum reached a maximum on Day 4 and remained elevated during the first 21 days of the BCG infection. Since FIM is synthesized and secreted by macrophages that have phagocytosed opsonized particles, it is highly probable that FIM occurring in serum originates from macrophages that have ingested BCG. The results of the present study led to the conclusion that FIM plays a role in the monocytosis developing during infection with BCG.
给小鼠静脉注射牛分枝杆菌（卡介苗）后，杆菌在脾脏、肝脏和肺部增殖，但腹腔仍保持无菌。在感染的前两周，血液单核细胞和肺泡巨噬细胞的数量增加，而腹腔巨噬细胞的数量保持不变。为了研究增加单核细胞生成的因子（FIM）在卡介苗感染期间单核细胞增多的调节中是否起作用，测定了感染期间不同时间间隔小鼠血清中该因子的活性。先前的研究表明，FIM通过对骨髓中单核母细胞和前单核细胞的有丝分裂活性产生影响来刺激单核细胞生成。血清中的FIM活性在第4天达到最大值，并在卡介苗感染的前21天保持升高。由于FIM是由吞噬了调理颗粒的巨噬细胞合成和分泌的，血清中出现的FIM很可能起源于摄取了卡介苗的巨噬细胞。本研究结果得出结论，FIM在卡介苗感染期间发生的单核细胞增多中起作用。
