Hamberger Anders, Stenhagen Gunnar
Department of Anatomy and Cell Biology, University of Göteborg, Göteborg, Sweden.
Neurochem Res. 2003 Feb;28(2):177-85. doi: 10.1023/a:1022364830421.
The aim of this study was to isolate a compound from blood plasma that inhibits intestinal diarrhea and that appears also to regulate fluid volumes in other organs. The isolation procedure included lipid extraction, liquid chromatography, and gas chromatography. The active substance was identified by mass spectrometry as erucamide (MW 337 Da). The biological effect was reproduced with authentic erucamide. Erucamide is a fatty acid amide, such as oleamide and anandamide, which modulate other physiological functions in a receptor-mediated fashion. All the exact biological functions of erucamide are as yet to be defined, but it is already known to stimulate angiogenesis. Erucamide concentrations were determined in body organs from the pig. The blood plasma level was 3 ng/g, and those of lung, kidney, liver, and brain were 12, 2.5, 1.0, and 0.5 ng/g, respectively. Erucamide was below detection level in the intestine, but is known to be present in the cerebrospinal fluid. In the rat, 3H-erucamide was accumulated in vivo into lung, liver, and spleen and in vitro into lung, liver, brain, and intestine. The in vitro uptake was time and temperature dependent, but not saturable.
本研究的目的是从血浆中分离出一种能抑制肠道腹泻且似乎还能调节其他器官液体量的化合物。分离程序包括脂质提取、液相色谱和气相色谱。通过质谱鉴定活性物质为芥酸酰胺（分子量337道尔顿）。用纯芥酸酰胺重现了生物学效应。芥酸酰胺是一种脂肪酸酰胺，如油酸酰胺和花生四烯酸乙醇胺，它们以受体介导的方式调节其他生理功能。芥酸酰胺所有确切的生物学功能尚未明确，但已知其能刺激血管生成。测定了猪体内各器官中的芥酸酰胺浓度。血浆水平为3纳克/克，肺、肾、肝和脑的水平分别为12、2.5、1.0和0.5纳克/克。芥酸酰胺在肠道中低于检测水平，但已知存在于脑脊液中。在大鼠体内，3H-芥酸酰胺在体内积聚于肺、肝和脾，在体外积聚于肺、肝、脑和肠道。体外摄取具有时间和温度依赖性，但不饱和。
