Gaspersic Rok, Stiblar-Martincic Draga, Osredkar Josko, Skaleric Uros
Department of Oral Medicine and Periodontology, Faculty of Medicine, University of Ljubljana, Slovenia.
J Periodontal Res. 2003 Apr;38(2):198-203. doi: 10.1034/j.1600-0765.2003.01395.x.
Proinflammatory cytokine tumor necrosis factor alpha (TNF-alpha) was found in inflamed periodontal tissues and many studies pointed to its significant role in development of periodontal disease. In this study, the influence of subcutaneously administered recombinant human TNF-alpha (rhTNF-alpha) on inflammatory reaction and periodontal breakdown in rats was analyzed during experimental periodontitis, induced by placing silk ligatures around the maxillary right second molar tooth. The rats were divided into two groups with five animals in each; the first group was infused subcutaneously with rhTNF-alpha via osmotic pumps for 2 weeks and the second group was infused with phosphate-buffered saline (PBS) in the same manner. Inflammatory reaction and periodontal breakdown was evaluated morphometrically on hematoxylin and eosin stained sections. Serum ionized calcium and inorganic phosphates were monitored colorimetrically. Serum calcium and phosphate levels were similar in rats receiving rhTNF-alpha and PBS. Ligation resulted in accelerated periodontal breakdown, while subcutaneous rhTNF-alpha administration by itself had no significant effect. Combined effect of subcutaneous rhTNF-alpha administration and ligation resulted in a significantly greater inflammatory reaction and periodontal breakdown then either treatment alone. We concluded that the subcutaneous administration of rhTNF-alpha accelerates the progression of experimental periodontitis in rats.
促炎细胞因子肿瘤坏死因子α（TNF-α）在炎症性牙周组织中被发现，许多研究指出其在牙周疾病发展中具有重要作用。在本研究中，分析了皮下注射重组人TNF-α（rhTNF-α）对丝线结扎上颌右侧第二磨牙诱发的实验性牙周炎大鼠炎症反应和牙周组织破坏的影响。将大鼠分为两组，每组五只；第一组通过渗透泵皮下注射rhTNF-α，持续2周，第二组以相同方式注射磷酸盐缓冲盐水（PBS）。在苏木精和伊红染色切片上进行形态计量学评估炎症反应和牙周组织破坏情况。比色法监测血清离子钙和无机磷酸盐。接受rhTNF-α和PBS的大鼠血清钙和磷酸盐水平相似。结扎导致牙周组织破坏加速，而单独皮下注射rhTNF-α本身无显著影响。皮下注射rhTNF-α与结扎的联合作用导致炎症反应和牙周组织破坏比单独任何一种治疗都显著更严重。我们得出结论，皮下注射rhTNF-α会加速大鼠实验性牙周炎的进展。
