[脂质蛋白复合物对重度烧伤大鼠肺血管通透性的影响]
[Effect of lipid protein complex on pulmonary vascular permeability in rats with severe burn].
作者信息
Fang Yong, Chen Yulin, Shou Yongming
机构信息
Burns and Plastic Surgery Department, Baogang Hospital, Shanghai Second Medical University, Shanghai 201900, China.
出版信息
Zhonghua Yi Xue Za Zhi. 2002 Nov 25;82(22):1561-4.
OBJECTIVE
To investigate the effect of lipid protein complex (LPC) from burn wound on the pulmonary vascular permeability.
METHODS
Fifty-two SD rats were made into 30%III degrees TBSA burn models. Six milliliters of peripheral blood were extracted from each rat 3 days after and LPC was isolated. Then 32 rats were killed. Their lungs and hearts were isolated to be made into experimental model of isolated perfused heart and lung and divided into 4 experimental groups perfused with different kinds of perfusion fluid containing LPC, serum from rats with burn, serum from normal rats, or perfusion fluid without above mentioned components (control group). Five minutes and 35 minutes after the perfusion, the lung weigh gain (LWG), fluid filtration coefficient (Kf) and pulmonary albumin permeability-surface area product (PS) were detected.
RESULTS
Five minutes after perfusion the LWG was 0.08 +/- 0.04 g in the burn serum group, and 0.07 +/- 0.04 g in the LPS group, both significantly higher than that in the control group (0.03 +/- 0.03, both P < 0.01). Thirty-five minutes after, the LWG was 0.56 +/- 0.12 g in the burn serum group, and 0.40 +/- 0.10 g in the LPS group, both significantly higher than that in the control group (0.26 +/- 0.08 g, P < 0.01) and with a significant difference between the burn serum group and LPC group (P < 0.05). PS was 7.6 +/- 1.3 x 10(-2) cm(3)/min x g DLW in the burn serum group, significantly higher than that in the control group (4.2 +/- 1.1 x 10(-2) cm(3)/min x g DLW, P < 0.01), and LPC group (4.6 +/- 1.2 2 x 10(-2) cm(3)/min x g DLW, P - 0.01), without a significant difference between the LPC and control group. Kf was 1.05 +/- 0.20 ml/min x kPa x g DLW in the burn serum group, and 0.89 +/- 0.13 ml/min x kPa x g DLW in the LPC group, both significantly higher than that in control group (0.32 +/- 0.09 ml/min x kPa x g DLW, P < 0.01) and with a significant difference between the burn serum group and LPC group (P < 0.05). No difference in LWG, PS, and Kf was found between normal serum group and control group.
CONCLUSION
LPC from burn serum induces pneumonedema in severe burn animal and may play a role in increasing vascular permeability to small molecules.
目的
探讨烧伤创面脂质蛋白复合物（LPC）对肺血管通透性的影响。
方法
将52只SD大鼠制成30%Ⅲ度体表面积烧伤模型。伤后3天从每只大鼠抽取6毫升外周血并分离出LPC。然后处死32只大鼠，分离其肺和心脏制成离体心肺灌注实验模型，分为4个实验组，分别用含LPC的不同灌注液、烧伤大鼠血清、正常大鼠血清或不含上述成分的灌注液（对照组）进行灌注。灌注后5分钟和35分钟，检测肺重量增加量（LWG）、液体滤过系数（Kf）和肺白蛋白通透表面积乘积（PS）。
结果
灌注后5分钟，烧伤血清组LWG为0.08±0.04克，LPC组为0.07±0.04克，均显著高于对照组（0.03±0.03，P均<0.01）。35分钟后，烧伤血清组LWG为0.56±0.12克，LPC组为0.40±0.10克，均显著高于对照组（0.26±0.08克，P<0.01），且烧伤血清组与LPC组之间有显著差异（P<0.05）。烧伤血清组PS为7.6±1.3×10⁻²立方厘米/分钟×克·DLW，显著高于对照组（4.2±1.1×10⁻²立方厘米/分钟×克·DLW，P<0.01）和LPC组（4.6±1.2×10⁻²立方厘米/分钟×克·DLW，P<0.01），LPC组与对照组之间无显著差异。烧伤血清组Kf为1.05±0.20毫升/分钟×千帕×克·DLW，LPC组为0.89±0.13毫升/分钟×千帕×克·DLW，均显著高于对照组（0.32±0.09毫升/分钟×千帕×克·DLW，P<0.01），且烧伤血清组与LPC组之间有显著差异（P<0.05）。正常血清组与对照组在LWG、PS和Kf方面无差异。
结论
烧伤血清中的LPC可导致严重烧伤动物发生肺水肿，可能在增加血管对小分子的通透性中起作用。
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