Komatsu H, Enzan K, Suzuki M
Department of Anesthesiology, Akita University School of Medicine.
Masui. 1994 Apr;43(4):534-7.
To investigate the effect of age on the filtration coefficient (Kf) in isolated rat perfused lungs, we divided 18 Sprague-Dawley rats randomly into 3 groups; 1) 5 wk of age, 150 +/- 1.5g, n = 6; 2) 10 wk of age, 320 +/- 18.8g, n = 6; and 3) 14 wk of age, 460 +/- 10.0g, n = 6. We used Krebs-Henseleit solution with 5% bovine serum albumin as the perfusate and maintained perfusion using constant pressure circuit system. We calculated the filtration coefficient in isolated rat perfused lungs in zone 3 conditions (pulmonary arterial pressure > pulmonary venous pressure > alveolar pressure) using gravimetric method. The filtration coefficients in rats of 14 wk of age were 3-fold higher than those in other two groups (Kf = 5.31 +/- 0.29 mg.min-1.cmH2O-1.g-1 in 5 wk of age, 5.38 +/- 0.49 mg.min-1.cmH2O-1.g-1 in 10 wk of age and 16.17 +/- 0.46 mg.min-1.cmH2O-1.g-1 in 14 wk of ages). Also, there were a large population of leukocytes in lung of 14 wk of age, but not in 5 wk and 10 wk of age. In addition, the filtration coefficients in 5 wk and 10 wk of age were of the same magnitude as in other investigators' reports. We conclude that rat in 10 wk of age is suitable for the evaluation of the pulmonary vascular permeability.
为研究年龄对离体大鼠灌注肺滤过系数（Kf）的影响，我们将18只Sprague-Dawley大鼠随机分为3组：1）5周龄，体重150±1.5g，n = 6；2）10周龄，体重320±18.8g，n = 6；3）14周龄，体重460±10.0g，n = 6。我们使用含5%牛血清白蛋白的Krebs-Henseleit溶液作为灌注液，并采用恒压循环系统维持灌注。我们采用重量法计算在3区条件（肺动脉压>肺静脉压>肺泡压）下离体大鼠灌注肺的滤过系数。14周龄大鼠的滤过系数比其他两组高3倍（5周龄时Kf = 5.31±0.29mg·min⁻¹·cmH₂O⁻¹·g⁻¹，10周龄时为5.38±0.49mg·min⁻¹·cmH₂O⁻¹·g⁻¹，14周龄时为16.17±0.46mg·min⁻¹·cmH₂O⁻¹·g⁻¹）。此外，14周龄大鼠肺中有大量白细胞，而5周龄和10周龄大鼠肺中则没有。另外，5周龄和10周龄大鼠的滤过系数与其他研究者报告中的数值相当。我们得出结论，10周龄大鼠适合用于评估肺血管通透性。
