In the presence of lipemia, the estimation of BR by diazo method is variable and hence unreliable. 2. The estimation of BR in lipemic plasma by the use of the A-O bilirubinometer yielded BR levels which were consistently lower than theoretical values. 3. By regression analysis of the percent error in BR estimation, (using the A-O bilirubinometer) and TG Concentrations, a straight line is obtained. Based upon this line, a correction factor for plasma BR concentration in the range of 5-25 mg/dl can be obtained if the degree of lipemia is known.