Noguchi Satoru, Tsukahara Toshifumi, Fujita Masako, Kurokawa Rumi, Tachikawa Masaji, Toda Tatsushi, Tsujimoto Atsumi, Arahata Kiichi, Nishino Ichizo
Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawahigashi, Kodaira, Tokyo 187-8502, Japan.
Hum Mol Genet. 2003 Mar 15;12(6):595-600.
We have developed a novel cDNA microarray encompassing 3500 genes expressed in skeletal muscle. With this system, we have performed the first study of gene expression in samples from individual patients. We analyzed muscle specimen from individuals with Duchenne muscular dystrophy to identify differences among patients. Among the variably expressed genes, we focused on the expression of the genes encoding HLA-related proteins, myosin light chains and troponin Ts as markers of muscle necrosis and regeneration. The expression patterns of these genes correlated with the severity of dystrophic changes on histological examination. Our cDNA microarray provides a new tool to investigate molecular muscle pathology.
我们开发了一种新型的cDNA微阵列，它涵盖了在骨骼肌中表达的3500个基因。利用这个系统，我们首次对个体患者的样本进行了基因表达研究。我们分析了杜兴氏肌营养不良症患者的肌肉标本，以确定患者之间的差异。在可变表达的基因中，我们重点关注编码HLA相关蛋白、肌球蛋白轻链和肌钙蛋白T的基因的表达，将其作为肌肉坏死和再生的标志物。这些基因的表达模式与组织学检查中营养不良性变化的严重程度相关。我们的cDNA微阵列提供了一种研究分子肌肉病理学的新工具。
