Feng Yuan, Yin Kaisheng, Wang Xiang, Xu Ying
Department of Geriatrics, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2002 Dec;25(12):736-8.
To establish an animal model of inflammatory pleural effusion and to investigate the role of batroxobin in preventing encapsulation of pleural effusion.
(1) Forty guinea pigs were divided into two groups: the animal model group received injection of 1% carrageenan (0.8 approximately 1.0 ml) in the right chest cavity; the control animals received injection of normal saline (1 ml). (2) Sixty guinea pigs were injected with 1% carrageenan (0.8 approximately 1.0 ml) in the right chest cavity, and then divided into a treatment group and a control group. Batroxobin (3 BU) was injected into the right chest cavity of the animals in the treatment group, while normal saline was used in the control group. The animals were killed at different time points.
In the animal model group, pleural effusion appeared in 24 hours and reached maximum amount within 2 approximately 3 days. Neutrophils accumulated in the effusion and pleural inflammation intensified at the same time. Fibrosis and pleural adhesion appeared by the seventh day, became prominent by the tenth day, and encapsulated pleurisy was evident by the fourteenth day. With batroxobin treatment, pleural effusion and inflammation attenuated. The fibrinogen (Fbg) level decreased, and the levels of tissue plasminogen activator (t-PA) and D-dimer increased in the effusion. No pleural encapsulation was observed.
Carrageenan was successfully used to establish an animal model of inflammatory pleural effusion in guinea pigs. Batroxobin was effective in reducing inflammation and pleural effusion and in preventing pleural fibrosis and encapsulation.
建立炎性胸腔积液动物模型，探讨巴曲酶在预防胸腔积液包裹中的作用。
（1）40只豚鼠分为两组：动物模型组在右侧胸腔注射1%角叉菜胶（0.8～1.0 ml）；对照组注射生理盐水（1 ml）。（2）60只豚鼠在右侧胸腔注射1%角叉菜胶（0.8～1.0 ml），然后分为治疗组和对照组。治疗组动物右侧胸腔注射巴曲酶（3 BU），对照组使用生理盐水。在不同时间点处死动物。
动物模型组在24小时出现胸腔积液，在2～3天内达到最大量。中性粒细胞在积液中积聚，同时胸膜炎症加剧。第7天出现纤维化和胸膜粘连，第10天变得明显，第14天出现包裹性胸膜炎。经巴曲酶治疗后，胸腔积液和炎症减轻。积液中纤维蛋白原（Fbg）水平降低，组织型纤溶酶原激活剂（t-PA）和D-二聚体水平升高。未观察到胸膜包裹。
角叉菜胶成功用于建立豚鼠炎性胸腔积液动物模型。巴曲酶可有效减轻炎症和胸腔积液，预防胸膜纤维化和包裹。
