Huang Juan, Zhou Dong, Tian Linyu, Wu Hongtao, Zhang Jie, Zhang Shangfu, Chen Huaiqing
Department of Neurology, West China Hospital of Sichuan University, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Jun;20(2):197-201.
To study the effect of batroxobin(DF-521) on atherosclerosis, we divided 50 Japanese big ear rabbits into control group and high-lipid group. After the atherosclerosis model was successfully established, the high-lipid rabbits were divided into 3 groups(placebo group, treatment group 1 and treatment group 2). Batroxobin was injected in the treatment groups, and saline was injected in placebo group and control group. Getting the aorta before, inter and after treatment, dyeing the lipid, endothelium, smooth muscle, collagen fibers of the vascular plaque(the elastic fibers are of autofluorescence), we observed them with the light microscope and laser scanning confocal microscope. From the results, we found that the atherosclerotic plaque in the treatment groups, tended to be static four weeks later, but there was no obvious difference between treatment group 1 and treatment group 2. These implied that batroxobin possessed the action of stabilizing the atherosclerotic plaque, but the dosage-effect was not clear and the principle needed more study.
为研究巴曲酶（DF-521）对动脉粥样硬化的影响，我们将50只日本大耳白兔分为对照组和高脂组。成功建立动脉粥样硬化模型后，将高脂兔分为3组（安慰剂组、治疗组1和治疗组2）。治疗组注射巴曲酶，安慰剂组和对照组注射生理盐水。在治疗前、治疗期间和治疗后获取主动脉，对血管斑块的脂质、内皮、平滑肌、胶原纤维进行染色（弹性纤维为自发荧光），用光镜和激光扫描共聚焦显微镜观察。结果发现，治疗组的动脉粥样硬化斑块在四周后趋于稳定，但治疗组1和治疗组2之间无明显差异。这表明巴曲酶具有稳定动脉粥样硬化斑块的作用，但量效关系不明确，其作用机制需要进一步研究。
