Keskes-Ammar L, Feki-Chakroun N, Rebai T, Sahnoun Z, Ghozzi H, Hammami S, Zghal K, Fki H, Damak J, Bahloul A
Laboratoire d'Histologie, Faculté de Médecine, Sfax, Tunisie.
Arch Androl. 2003 Mar-Apr;49(2):83-94. doi: 10.1080/01485010390129269.
Numerous studies have reported beneficial effects of antioxidant drugs on semen quality, but there is no well-defined therapeutical protocol in male infertility. This study aimed to test the effects of vitamin E and selenium supplementation on lipid peroxidation and on sperm parameters. The study included 54 voluntary and infertile men who produced semen samples for spermiogram and for spectrophotometric measurement of a lipid peroxidation marker, the malondialdehyde (MDA), and produced blood samples for high-performance liquid chromatography assessment of serum vitamin E level. The trial was randomized and open. Twenty-eight men were supplemented daily by vitamin E (400 mg) and selenium (225 microg), during 3 months. The remaining 26 patients received vitamin B (4,5 g/day) for the same duration. Only 20 patients achieved their treatment and returned for control analysis. MDA concentrations in sperm were much less than in seminal plasma and motility and viability were inversely correlated with semen MDA levels. In contrast to vitamin B supplementation, vitamin E and selenium supplementation produced a significant decrease in MDA concentrations and an improvement of sperm motility. The results confirm the protective and beneficial effects of vitamin E and selenium on semen quality and advocate their use in male infertility treatment.
许多研究报告了抗氧化剂药物对精液质量的有益作用，但男性不育症尚无明确的治疗方案。本研究旨在测试补充维生素E和硒对脂质过氧化及精子参数的影响。该研究纳入了54名自愿参与的不育男性，他们提供精液样本用于精子图谱分析及脂质过氧化标志物丙二醛（MDA）的分光光度测量，并提供血液样本用于血清维生素E水平的高效液相色谱评估。试验为随机开放试验。28名男性在3个月内每日补充维生素E（400毫克）和硒（225微克）。其余26名患者在相同时间段内每日服用维生素B（4.5克）。只有20名患者完成治疗并返回进行对照分析。精子中的MDA浓度远低于精浆中的浓度，且精子活力和存活率与精液MDA水平呈负相关。与补充维生素B相比，补充维生素E和硒可使MDA浓度显著降低，并改善精子活力。结果证实了维生素E和硒对精液质量的保护和有益作用，并提倡在男性不育治疗中使用它们。
