通过添加VlsE和源自加氏疏螺旋体的DbpA同源物显著改进重组疏螺旋体特异性免疫球蛋白G免疫印迹试验用于早期神经莱姆病的诊断。
Significant improvement of the recombinant Borrelia-specific immunoglobulin G immunoblot test by addition of VlsE and a DbpA homologue derived from Borrelia garinii for diagnosis of early neuroborreliosis.
作者信息
Schulte-Spechtel Ulrike, Lehnert Gisela, Liegl Gaby, Fingerle Volker, Heimerl Christiane, Johnson Barbara J B, Wilske Bettina
机构信息
Max von Pettenkofer-Institut für Medizinische Mikrobiologie und Hygiene der Ludwig-Maximilians-Universität München, D-80336 Munich, Germany.
出版信息
J Clin Microbiol. 2003 Mar;41(3):1299-303. doi: 10.1128/JCM.41.3.1299-1303.2003.
Abstract
We investigated whether the recombinant Borrelia Western blot test previously described (B. Wilske, C. Habermann, V. Fingerle, B. Hillenbrand, S. Jauris-Heipke, G. Lehnert, I. Pradel, D. Rössler, and U. Schulte-Spechtel, Med. Microbiol. Immunol. 188:139-144, 1999) can be improved by the addition of VlsE and additional DbpA and OspC homologues. By using a panel of sera from 36 neuroborreliosis patients and 67 control patients, the diagnostic sensitivity of the recombinant immunoblot test was significantly increased (86.1% versus 52.7%) without loss of specificity and was higher (86.1% versus 63.8%) than that of the conventional whole-cell lysate immunoblot test (U. Hauser, G. Lehnert, R. Lobentanzer, and B. Wilske, J. Clin. Microbiol. 35:1433-1444, 1997). Improvement was mainly due to the presence of VlsE and DbpA.
摘要
我们研究了先前描述的重组疏螺旋体免疫印迹试验（B. Wilske、C. Habermann、V. Fingerle、B. Hillenbrand、S. Jauris-Heipke、G. Lehnert、I. Pradel、D. Rössler和U. Schulte-Spechtel，《医学微生物学与免疫学》188:139 - 144，1999年）通过添加VlsE以及额外的DbpA和OspC同源物是否能够得到改进。通过使用一组来自36例神经莱姆病患者和67例对照患者的血清，重组免疫印迹试验的诊断敏感性显著提高（从52.7%提高到86.1%），且特异性未降低，并且高于传统全细胞裂解物免疫印迹试验（U. Hauser、G. Lehnert、R. Lobentanzer和B. Wilske，《临床微生物学杂志》35:1433 - 1444，1997年）（从63.8%提高到86.1%）。改进主要归因于VlsE和DbpA的存在。
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