Clinical Department of Laboratory Medicine and National Reference Center for Borrelia Burgdorferi, University Hospitals Leuven, Leuven, Belgium.
Department of Microbiology, Immunology and Transplantation, Laboratory of Clinical Bacteriology and Mycology, KU Leuven, Leuven, Belgium.
Eur J Clin Microbiol Infect Dis. 2022 Jan;41(1):155-161. doi: 10.1007/s10096-021-04343-x. Epub 2021 Sep 13.
For diagnosis of neuroborreliosis, calculation of the antibody index, based on Euroimmun Anti-Borrelia plus VlsE ELISA was compared to Virotech Borrelia Europe plus TpN17 immunoblot-based detection of Borrelia-specific intrathecal antibody production. CXCL13 results in cerebrospinal fluid were used to evaluate discordant results. A total of 64 serum/CSF pairs were analysed. Patients were classified according to European Federation of Neurological Societies criteria incorporating Virotech results. For the Euroimmun assay, a sensitivity of 100% and specificity of 94% was found. Agreement between the both tests was almost perfect (κ 0.81). Both methods are appropriate for the detection of Borrelia-specific intrathecal antibody production.
为了诊断神经莱姆病，将基于 Euroimmun Anti-Borrelia plus VlsE ELISA 的抗体指数计算与 Virotech Borrelia Europe plus TpN17 免疫印迹法检测针对 Borrelia 的鞘内抗体产生进行了比较。使用 CXCL13 结果评估了不一致的结果。共分析了 64 对血清/CSF 样本。根据包含 Virotech 结果的欧洲神经病学会联合会标准对患者进行分类。对于 Euroimmun 检测，发现敏感性为 100%，特异性为 94%。两种检测方法的一致性几乎为完美（κ 0.81）。两种方法均适用于检测 Borrelia 特异性鞘内抗体产生。
