Sambuk E V, Popova Iu G, Fizikova A Iu, Padkina M V
Biological Institute, St. Petersburg State University, St. Petersburg, 198904 Russia.
Genetika. 2003 Aug;39(8):1039-45.
The cyclin-dependent phosphoprotein kinase Pho85p is involved in the regulation of metabolism and cell cycle in the yeast Saccharomyces cerevisiae. It is known that mutations in the PHO85 gene lead to constitutive synthesis of Pho5p acidic phosphatase, a delay in cell growth on media containing nonfermentable carbon sources, sensitivity to high temperature, and other phenotypic effects. A lack of growth at 37 degrees C and on a medium with alcohol as the carbon source was shown to be associated with the rapid accumulation of nuclear ts and mitochondrial [rho-] mutations occurring in the background of gene PHO85 inactivation. Thus, Pho85p seems to play an important role in the maintenance of yeast genome stability.
细胞周期蛋白依赖性磷酸蛋白激酶Pho85p参与酿酒酵母的代谢和细胞周期调控。已知PHO85基因突变会导致Pho5p酸性磷酸酶的组成型合成、在含有非发酵性碳源的培养基上细胞生长延迟、对高温敏感以及其他表型效应。已表明在37摄氏度和以酒精作为碳源的培养基上缺乏生长与在基因PHO85失活背景下发生的核ts和线粒体[rho-]突变的快速积累有关。因此，Pho85p似乎在维持酵母基因组稳定性中发挥重要作用。
