Gambari Roberto, Borgatti Monica, Altomare Luigi, Manaresi Nicolò, Medoro Gianni, Romani Aldo, Tartagni Marco, Guerrieri Roberto
Biotechnology Center, University of Ferrara, Italy, Via Fossato di Mortara, 44100 Ferrara, Italy.
Technol Cancer Res Treat. 2003 Feb;2(1):31-40. doi: 10.1177/153303460300200105.
The recent development of advanced analytical and bioseparation methodologies based on microarrays and biosensors is one of the strategic objectives of the so-called post-genomic. In this field, the development of microfabricated devices could bring new opportunities in several application fields, such as predictive oncology, diagnostics and anti-tumor drug research. The so called "Laboratory-on-a-chip technology", involving miniaturisation of analytical procedures, is expected to enable highly complex laboratory testing to move from the central laboratory into non-laboratory settings. The main advantages of Lab-on-a-chip devices are integration of multiple steps of different analytical procedures, large variety of applications, sub-microliter consumption of reagents and samples, and portability. One of the requirement for new generation Lab-on-a-chip devices is the possibility to be independent from additional preparative/analytical instruments. Ideally, Lab-on-a-chip devices should be able to perform with high efficiency and reproducibility both actuating and sensing procedures. In this review, we discuss applications of dielectrophoretic(DEP)-based Lab-on-a-chip devices to cancer research. The theory of dielectrophoresis as well as the description of several devices, based on spiral-shaped, parallel and arrayed electrodes are here presented. In addition, in this review we describe manipulation of cancer cells using advanced DEP-based Lab-on-a-chip devices in the absence of fluid flow and with the integration of both actuating and sensing procedures.
基于微阵列和生物传感器的先进分析和生物分离方法的最新发展是所谓后基因组学的战略目标之一。在这一领域，微制造设备的发展可能会在多个应用领域带来新机遇，如预测性肿瘤学、诊断学和抗肿瘤药物研究。所谓的“芯片实验室技术”，涉及分析程序的小型化，有望使高度复杂的实验室检测从中心实验室转移到非实验室环境中。芯片实验室设备的主要优点包括不同分析程序多个步骤的集成、多种应用、试剂和样品的亚微升消耗以及便携性。新一代芯片实验室设备的要求之一是能够独立于额外的制备/分析仪器。理想情况下，芯片实验室设备应能够高效且可重复地执行驱动和传感程序。在本综述中，我们讨论基于介电电泳（DEP）的芯片实验室设备在癌症研究中的应用。本文介绍了介电电泳理论以及基于螺旋形、平行和阵列电极的几种设备。此外，在本综述中，我们描述了在无流体流动且集成了驱动和传感程序的情况下，使用先进的基于DEP的芯片实验室设备对癌细胞进行操控。
