用于生物粒子操控的介电电泳。

Dielectrophoresis for bioparticle manipulation.

作者信息

Qian Cheng, Huang Haibo, Chen Liguo, Li Xiangpeng, Ge Zunbiao, Chen Tao, Yang Zhan, Sun Lining

机构信息

Robotics and Microsystems Center, College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215000, China.

出版信息

Int J Mol Sci. 2014 Oct 10;15(10):18281-309. doi: 10.3390/ijms151018281.

As an ideal method to manipulate biological particles, the dielectrophoresis (DEP) technique has been widely used in clinical diagnosis, disease treatment, drug development, immunoassays, cell sorting, etc. This review summarizes the research in the field of bioparticle manipulation based on DEP techniques. Firstly, the basic principle of DEP and its classical theories are introduced in brief; Secondly, a detailed introduction on the DEP technique used for bioparticle manipulation is presented, in which the applications are classified into five fields: capturing bioparticles to specific regions, focusing bioparticles in the sample, characterizing biomolecular interaction and detecting microorganism, pairing cells for electrofusion and separating different kinds of bioparticles; Thirdly, the effect of DEP on bioparticle viability is analyzed; Finally, the DEP techniques are summarized and future trends in bioparticle manipulation are suggested.

作为一种操纵生物粒子的理想方法，介电泳（DEP）技术已广泛应用于临床诊断、疾病治疗、药物开发、免疫分析、细胞分选等领域。本综述总结了基于DEP技术的生物粒子操纵领域的研究。首先，简要介绍了DEP的基本原理及其经典理论；其次，详细介绍了用于生物粒子操纵的DEP技术，其中应用分为五个领域：将生物粒子捕获到特定区域、使生物粒子在样品中聚焦、表征生物分子相互作用和检测微生物、将细胞配对进行电融合以及分离不同种类的生物粒子；第三，分析了DEP对生物粒子活力的影响；最后，总结了DEP技术并提出了生物粒子操纵的未来趋势。