Vilas N N, Bell R R, Draper H H
J Nutr. 1976 May;106(5):589-96. doi: 10.1093/jn/106.5.589.
The influence of dietary peroxides, vitamin E and selenium on glutathione peroxidase (GSH-Px) activity in the gastrointestinal tract of the rat was investigated. Feeding 7% oxidized stripped corn oid (peroxide value 1,000) in a diet adequate in selenium and vitamin E increased the specific activity of GSH-Px in the stomach mucosa. Feeding oxidized oil produced an increase in the wet weight of the intestinal mucosa which was associated with a decrease in the specific activity of the enzyme. Total GSH-Px activity in the intestinal mucosa was unchanged or moderately increased. These changes were unaffected by the presence of vitamin E in the diet. Dietary peroxides had no effect on GSH-Px activity in the plasma or in the perirenal and paraepididymal adipose tissues. Subacute vitamin E deficiency had no consistent effect on the activity of the enzyme in several tissues examined. In rats fed a Se deficient diet glutathione peroxidase activity decreased markedly in most tissues but only slightly in the intestinal mucosa. The moderate decrease in the intestine may be explained by the accessibility of residual dietary Se to the mucosal cells. The role of Se in the detoxification of peroxides in foods and the response of gastrointestinal GSH-Px to dietary peroxides are discussed.
研究了膳食过氧化物、维生素E和硒对大鼠胃肠道谷胱甘肽过氧化物酶（GSH-Px）活性的影响。在硒和维生素E充足的日粮中添加7%氧化脱壳玉米油（过氧化值1000），可提高胃黏膜中GSH-Px的比活性。饲喂氧化油会使肠黏膜湿重增加，同时酶的比活性降低。肠黏膜中GSH-Px的总活性未变或略有增加。日粮中维生素E的存在不影响这些变化。膳食过氧化物对血浆、肾周和附睾旁脂肪组织中的GSH-Px活性没有影响。亚急性维生素E缺乏对所检测的几种组织中的酶活性没有一致的影响。在饲喂缺硒日粮的大鼠中，大多数组织中的谷胱甘肽过氧化物酶活性显著降低，但肠黏膜中仅略有降低。肠道中的适度降低可能是由于残留日粮硒可被黏膜细胞利用。讨论了硒在食物过氧化物解毒中的作用以及胃肠道GSH-Px对膳食过氧化物的反应。
