Wang Xiao-Zhong, Zhang Li-Juan, Li Dan, Huang Yue-Hong, Chen Zhi-Xin, Li Bin
Department of Gastroenterology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, Fujian Province, China.
World J Gastroenterol. 2003 Mar;9(3):539-43. doi: 10.3748/wjg.v9.i3.539.
To study the effects of transmitters ET, AgII, PGI(2), CGRP and GG on experimental rat hepatic fibrosis and the antifibrogenic effects of IL-10.
One hundred SD rats were randomly divided into 3 groups: control group (N): intraperitoneal injection with saline 2 ml.kg(-1) twice a week; the fibrogenesis group (C): intraperitoneal injection with 50 % CCl(4) 2 ml.kg(-1) twice a week; IL-10 treated group (E): besides same dosage of CCl(4) given, intraperitoneal injection with IL-10 4 ug.kg(-1) from the third week. In the fifth, the seventh and the ninth week, rats in three groups were selected randomly to collect plasma and liver tissues. The levels of ET, AgII, PGI(2), CGRP and GG were assayed by radioimmunoassay (RIA). The liver fibrosis was observed with silver staining.
The hepatic fibrosis was developed with the increase of the injection frequency of CCl(4). The ET, AgII, PGI(2), CGRP and GG levels in serum of group N were 71.84+/-60.2 ng.L(-1), 76.21+/-33.3 ng/L, 313.03+/-101.71 ng/L, 61.97+/-21.4 ng/L and 33.62+/-14.37 ng/L, respectively; the levels of them in serum of group C were 523.30+/-129.3 ng/L, 127.24+/-50.0 ng/L, 648.91+/-357.29 ng/L, 127.15+/-62.0 ng/L and 85.26+/-51.83 ng/L, respectively; the levels of them in serum of group E were 452.52+/-99.5 ng/L, 90.60+/-44.7 ng/L, 475.57+/-179.70 ng/L, 102.2+/-29.7 ng/L and 38.05+/-19.94 ng/L, respectively. The histological examination showed that the degrees of the rats liver fibrosis in group E were lower than those in group C.
The transmitters ET, AgII, PGI(2), CGRP and GG play a significant role in the rat hepatic fibrosis induced by CCl(4). IL-10 has the antagonistic action on these transmitters and can relieve the degree of the liver fibrosis.
研究递质内皮素（ET）、血管紧张素II（AgII）、前列环素（PGI₂）、降钙素基因相关肽（CGRP）和甘胆酸（GG）对实验性大鼠肝纤维化的影响以及白细胞介素-10（IL-10）的抗纤维化作用。
将100只SD大鼠随机分为3组：对照组（N）：每周两次腹腔注射2 ml·kg⁻¹生理盐水；纤维化形成组（C）：每周两次腹腔注射50%四氯化碳（CCl₄）2 ml·kg⁻¹；IL-10治疗组（E）：除给予相同剂量的CCl₄外，从第3周起每周两次腹腔注射IL-10 4 μg·kg⁻¹。在第5、7和9周，随机选取3组大鼠采集血浆和肝组织。采用放射免疫分析法（RIA）检测ET、AgII、PGI₂、CGRP和GG的水平。用银染色观察肝纤维化情况。
随着CCl₄注射次数的增加，肝纤维化逐渐发展。N组血清中ET、AgII、PGI₂、CGRP和GG水平分别为71.84±60.2 ng·L⁻¹、76.21±33.3 ng/L、313.03±101.71 ng/L、61.97±21.4 ng/L和33.62±14.37 ng/L；C组血清中它们的水平分别为523.30±129.3 ng/L、127.24±50.0 ng/L、648.91±357.29 ng/L、127.15±62.0 ng/L和85.26±51.83 ng/L；E组血清中它们的水平分别为452.52±99.5 ng/L、90.60±44.7 ng/L、475.57±179.70 ng/L、102.2±29.7 ng/L和38.05±19.94 ng/L。组织学检查显示，E组大鼠肝纤维化程度低于C组。
递质ET、AgII、PGI₂、CGRP和GG在CCl₄诱导的大鼠肝纤维化中起重要作用。IL-10对这些递质具有拮抗作用，可减轻肝纤维化程度。
