Kashiwaba Mitsuhiro, Katsura Koji, Ohnishi Motoko, Sasaki Mutsuo, Tanaka Hiromitsu, Nishimune Yoshitake, Kobayashi Takayasu, Tamura Shinri
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan.
FEBS Lett. 2003 Mar 13;538(1-3):197-202. doi: 10.1016/s0014-5793(03)00153-4.
In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Czeta, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Czeta and PP2Calpha was 22%. On Northern blot analysis PP2Czeta was found to be expressed specifically in the testicular germ cells. PP2Czeta expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Czeta exhibits its specific role through its SUMO-induced recruitment to UBC9.
在本研究中，我们克隆了小鼠蛋白磷酸酶2C家族的一个新成员PP2Cζ，它由507个氨基酸组成，具有独特的N端区域。PP2Cζ与PP2Cα之间氨基酸序列的总体相似性为22%。Northern印迹分析发现PP2Cζ在睾丸生殖细胞中特异性表达。在COS7细胞中表达的PP2Cζ能够与泛素结合酶9（UBC9）结合，并且通过共表达小泛素相关修饰物-1（SUMO-1）增强这种结合，这表明PP2Cζ通过其SUMO诱导的向UBC9的募集发挥其特定作用。
