一种新型蛋白磷酸酶2C家族成员（PP2Cζ）能够与泛素结合酶9相互作用。
A novel protein phosphatase 2C family member (PP2Czeta) is able to associate with ubiquitin conjugating enzyme 9.
作者信息
Kashiwaba Mitsuhiro, Katsura Koji, Ohnishi Motoko, Sasaki Mutsuo, Tanaka Hiromitsu, Nishimune Yoshitake, Kobayashi Takayasu, Tamura Shinri
机构信息
Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan.
出版信息
FEBS Lett. 2003 Mar 13;538(1-3):197-202. doi: 10.1016/s0014-5793(03)00153-4.
In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Czeta, which is composed of 507 amino acids and has a unique N-terminal region. The overall similarity of the amino acid sequence between PP2Czeta and PP2Calpha was 22%. On Northern blot analysis PP2Czeta was found to be expressed specifically in the testicular germ cells. PP2Czeta expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co-expression of small ubiquitin-related modifier-1 (SUMO-1), suggesting that PP2Czeta exhibits its specific role through its SUMO-induced recruitment to UBC9.
在本研究中，我们克隆了小鼠蛋白磷酸酶2C家族的一个新成员PP2Cζ，它由507个氨基酸组成，具有独特的N端区域。PP2Cζ与PP2Cα之间氨基酸序列的总体相似性为22%。Northern印迹分析发现PP2Cζ在睾丸生殖细胞中特异性表达。在COS7细胞中表达的PP2Cζ能够与泛素结合酶9（UBC9）结合，并且通过共表达小泛素相关修饰物-1（SUMO-1）增强这种结合，这表明PP2Cζ通过其SUMO诱导的向UBC9的募集发挥其特定作用。
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