蛋白磷酸酶2C家族新成员PP2Ceta的分子克隆

Molecular cloning of PP2Ceta, a novel member of the protein phosphatase 2C family.

作者信息

Komaki Ken-ichiro, Katsura Koji, Ohnishi Motoko, Guang Li Ming, Sasaki Masato, Watanabe Makoto, Kobayashi Takayasu, Tamura Shinri

机构信息

Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.

出版信息

Biochim Biophys Acta. 2003 Nov 30;1630(2-3):130-7. doi: 10.1016/j.bbaexp.2003.09.004.

DOI:10.1016/j.bbaexp.2003.09.004

PMID:14654243

Abstract

We have cloned a novel member of the mouse protein phosphatase 2C family, PP2Ceta. Sequence analysis suggests that PP2Ceta, PP2Czeta and NERPP-2C constitute a unique subgroup of the PP2C family. PP2Ceta had extremely low activity against alpha-casein compared with PP2Calpha and was localized mainly in cell nuclei, suggesting that PP2Ceta dephosphorylates a unique nuclear protein(s) in the cells.

摘要

我们克隆了小鼠蛋白磷酸酶2C家族的一个新成员PP2Ceta。序列分析表明，PP2Ceta、PP2Czeta和NERPP - 2C构成了PP2C家族的一个独特亚组。与PP2Calpha相比，PP2Ceta对α - 酪蛋白的活性极低，且主要定位于细胞核，这表明PP2Ceta使细胞中一种独特的核蛋白去磷酸化。