Henneke Ghislaine, Koundrioukoff Stéphane, Hübscher Ulrich
Institute of Veterinary Biochemistry and Molecular Biology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland.
EMBO Rep. 2003 Mar;4(3):252-6. doi: 10.1038/sj.embor.embor774.
DNA replication is carried out by the replisome, which includes several proteins that are targets of cell-cycle-regulated kinases. The phosphorylation of proteins such as replication protein A, DNA polymerase-alpha and -delta, replication factor C, flap endonuclease 1 and DNA ligase I leads to their inactivation, suggesting that phosphorylation is important in the prevention of re-replication. Moreover, the phosphorylation of several of these replication proteins has been shown to block their association with the 'moving platform'-proliferating cell nuclear antigen. Therefore, phosphorylation seems to be a crucial regulator of replisome assembly and DNA replication, although its precise role in these processes remains to be clarified.
DNA复制由复制体完成，复制体包含几种蛋白质，这些蛋白质是细胞周期调控激酶的作用靶点。诸如复制蛋白A、DNA聚合酶α和δ、复制因子C、瓣状核酸内切酶1和DNA连接酶I等蛋白质的磷酸化会导致它们失活，这表明磷酸化在防止重新复制中很重要。此外，已证明这些复制蛋白中的几种磷酸化会阻止它们与“移动平台”——增殖细胞核抗原的结合。因此，磷酸化似乎是复制体组装和DNA复制的关键调节因子，尽管其在这些过程中的精确作用仍有待阐明。
