参与组氨酸和色氨酸生物合成的一种假定的类古异构体酶的出现。
Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis.
作者信息
Barona-Gómez Francisco, Hodgson David A
机构信息
Department of Biological Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK.
出版信息
EMBO Rep. 2003 Mar;4(3):296-300. doi: 10.1038/sj.embor.embor771.
Abstract
We report the occurrence of an isomerase with a putative (betaalpha)(8)-barrel structure involved in both histidine and trypto-phan biosynthesis in Streptomyces coelicolor A3(2) and Mycobacterium tuberculosis HR37Rv. Deletion of a hisA homologue (SCO2050) putatively encoding N'-[(5'-phosphoribosyl)-formimino]-5 amino-imidazole-4-carboxamide ribonucleotide isomerase from the chromosome of S. coelicolor A3(2) generated a double auxotrophic mutant for histidine and tryptophan. The bifunctional gene SCO2050 and its orthologue Rv1603 from M. tuberculosis complemented both hisA and trpF mutants of Escherichia coli. Expression of the E. coli trpF gene in the S. coelicolor mutant only complemented the tryptophan auxo-trophy, and the hisA gene only complemented the histidine auxotrophy. The discovery of this enzyme, which has a broad-substrate specificity, has implications for the evolution of metabolic pathways and may prove to be important for understanding the evolution of the (betaalpha)(8)-barrels.
摘要
我们报道了在天蓝色链霉菌A3(2)和结核分枝杆菌HR37Rv中存在一种具有假定的(betaalpha)(8)-桶状结构的异构酶，该异构酶参与组氨酸和色氨酸的生物合成。从天蓝色链霉菌A3(2)染色体上缺失一个假定编码N'-[(5'-磷酸核糖基)-甲脒基]-5-氨基咪唑-4-甲酰胺核糖核苷酸异构酶的hisA同源物(SCO2050)，产生了一个组氨酸和色氨酸双营养缺陷型突变体。来自结核分枝杆菌的双功能基因SCO2050及其直向同源物Rv1603补充了大肠杆菌的hisA和trpF突变体。在天蓝色链霉菌突变体中表达大肠杆菌trpF基因仅补充了色氨酸营养缺陷，而hisA基因仅补充了组氨酸营养缺陷。这种具有广泛底物特异性的酶的发现，对代谢途径的进化具有启示意义，并且可能被证明对理解(betaalpha)(8)-桶状结构的进化很重要。
相似文献
1
Occurrence of a putative ancient-like isomerase involved in histidine and tryptophan biosynthesis.参与组氨酸和色氨酸生物合成的一种假定的类古异构体酶的出现。
EMBO Rep. 2003 Mar;4(3):296-300. doi: 10.1038/sj.embor.embor771.
2
Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates.组氨酸和色氨酸生物合成过程中的两种（βα）（8）桶状酶具有相似的反应机制以及保护其不稳定底物的共同策略。
Biochemistry. 2002 Oct 8;41(40):12032-42. doi: 10.1021/bi026092h.
3
Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways.对一种（β-α）8桶状酶进行定向进化，以催化两条不同代谢途径中的相关反应。
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9925-30. doi: 10.1073/pnas.160255397.
4
Expression, purification and preliminary crystallographic analysis of phosphoribosyl isomerase (PriA) from Streptomyces coelicolor.天蓝色链霉菌磷酸核糖异构酶（PriA）的表达、纯化及初步晶体学分析
Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):534-6. doi: 10.1107/S0907444903028877. Epub 2004 Feb 25.
5
Evolution of substrate specificity in a recipient's enzyme following horizontal gene transfer.在水平基因转移后，受体酶的底物特异性的演变。
Mol Biol Evol. 2013 Sep;30(9):2024-34. doi: 10.1093/molbev/mst115. Epub 2013 Jun 25.
6
Molecular cloning and characterization of the gene encoding N'-[(5'-phosphoribosyl)-formimino]-5-aminoimidazole-4-carboxamide ribonucleotide (BBM II) isomerase from Arabidopsis thaliana.来自拟南芥的N'-[(5'-磷酸核糖基)-甲脒基]-5-氨基咪唑-4-甲酰胺核糖核苷酸（BBM II）异构酶编码基因的分子克隆与特性分析
Mol Gen Genet. 1998 Aug;259(2):216-23. doi: 10.1007/s004380050807.
7
Two-step Ligand Binding in a (βα)8 Barrel Enzyme: SUBSTRATE-BOUND STRUCTURES SHED NEW LIGHT ON THE CATALYTIC CYCLE OF HisA.(βα)8桶状酶中的两步配体结合：底物结合结构为HisA催化循环提供新线索
J Biol Chem. 2015 Oct 9;290(41):24657-68. doi: 10.1074/jbc.M115.678086. Epub 2015 Aug 20.
8
Co-occurrence of analogous enzymes determines evolution of a novel (βα)8-isomerase sub-family after non-conserved mutations in flexible loop.类似酶的共现决定了柔性环中发生非保守突变后一个新的(βα)8异构酶亚家族的进化。
Biochem J. 2016 May 1;473(9):1141-52. doi: 10.1042/BJ20151271. Epub 2016 Feb 29.
9
Interconverting the catalytic activities of (betaalpha)(8)-barrel enzymes from different metabolic pathways: sequence requirements and molecular analysis.不同代谢途径中（βα）8桶状酶催化活性的相互转换：序列要求与分子分析
J Mol Biol. 2004 Apr 2;337(4):871-9. doi: 10.1016/j.jmb.2004.01.062.
10
Insights into the evolution of enzyme substrate promiscuity after the discovery of (βα)₈ isomerase evolutionary intermediates from a diverse metagenome.从多样的宏基因组中发现(βα)₈异构酶进化中间体后对酶底物选择性进化的见解。
BMC Evol Biol. 2015 Jun 10;15:107. doi: 10.1186/s12862-015-0378-1.
引用本文的文献
1
PhyloString: A web server designed to identify, visualize, and evaluate functional relationships between orthologous protein groups across different phylogenetic lineages.PhyloString：一个旨在识别、可视化和评估不同系统发育谱系中直系同源蛋白组之间功能关系的网络服务器。
PLoS One. 2024 Jan 26;19(1):e0297010. doi: 10.1371/journal.pone.0297010. eCollection 2024.
2
Complex Loop Dynamics Underpin Activity, Specificity, and Evolvability in the (βα) Barrel Enzymes of Histidine and Tryptophan Biosynthesis.复杂的环动力学支撑组氨酸和色氨酸生物合成的（βα）桶状酶的活性、特异性和可进化性。
JACS Au. 2022 Apr 4;2(4):943-960. doi: 10.1021/jacsau.2c00063. eCollection 2022 Apr 25.
3
Cross-kingdom expression of synthetic genetic elements promotes discovery of metabolites in the human microbiome.跨物种表达合成遗传元件可促进人类微生物组代谢产物的发现。
Cell. 2022 Apr 28;185(9):1487-1505.e14. doi: 10.1016/j.cell.2022.03.008. Epub 2022 Apr 1.
4
Mutational Pathways and Trade-Offs Between HisA and TrpF Functions: Implications for Evolution via Gene Duplication and Divergence.组氨酸A和色氨酸F功能之间的突变途径与权衡：对基因复制和分化进化的启示
Front Microbiol. 2020 Oct 14;11:588235. doi: 10.3389/fmicb.2020.588235. eCollection 2020.
5
The Role of Gene Elongation in the Evolution of Histidine Biosynthetic Genes.基因延伸在组氨酸生物合成基因进化中的作用。
Microorganisms. 2020 May 13;8(5):732. doi: 10.3390/microorganisms8050732.
6
How enzyme promiscuity and horizontal gene transfer contribute to metabolic innovation.酶的多功能性和水平基因转移如何促进代谢创新。
FEBS J. 2020 Apr;287(7):1323-1342. doi: 10.1111/febs.15185. Epub 2020 Jan 10.
7
Evolutionary convergence in the biosyntheses of the imidazole moieties of histidine and purines.组氨酸和嘌呤中咪唑部分生物合成的进化趋同。
PLoS One. 2018 Apr 26;13(4):e0196349. doi: 10.1371/journal.pone.0196349. eCollection 2018.
8
Endoglucanase activity at a second site in triosephosphate isomerase-Promiscuity or compensation for a metabolic handicap?磷酸丙糖异构酶第二个位点的内切葡聚糖酶活性——是催化混杂性还是对代谢缺陷的补偿？
FEBS Open Bio. 2017 Jul 11;7(8):1126-1143. doi: 10.1002/2211-5463.12249. eCollection 2017 Aug.
9
Multifunctional enzymes from reduced genomes - model proteins for simple primordial metabolism?来自简化基因组的多功能酶——简单原始代谢的模型蛋白？
Mol Microbiol. 2017 Aug;105(4):505-507. doi: 10.1111/mmi.13742. Epub 2017 Jul 14.
10
AGeNNT: annotation of enzyme families by means of refined neighborhood networks.AGeNNT：通过精细邻域网络对酶家族进行注释。
BMC Bioinformatics. 2017 May 25;18(1):274. doi: 10.1186/s12859-017-1689-6.
本文引用的文献
1
PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin.聚合酶链反应（PCR）靶向的链霉菌基因置换鉴定出倍半萜类土壤气味物质土臭素生物合成所需的一个蛋白质结构域。
Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1541-6. doi: 10.1073/pnas.0337542100. Epub 2003 Jan 31.
2
Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates.组氨酸和色氨酸生物合成过程中的两种（βα）（8）桶状酶具有相似的反应机制以及保护其不稳定底物的共同策略。
Biochemistry. 2002 Oct 8;41(40):12032-42. doi: 10.1021/bi026092h.
3
One fold with many functions: the evolutionary relationships between TIM barrel families based on their sequences, structures and functions.一型多用：基于序列、结构和功能的TIM桶状家族之间的进化关系
J Mol Biol. 2002 Aug 30;321(5):741-65. doi: 10.1016/s0022-2836(02)00649-6.
4
Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2).模式放线菌天蓝色链霉菌A3(2)的全基因组序列
Nature. 2002 May 9;417(6885):141-7. doi: 10.1038/417141a.
5
Divergent evolution of (betaalpha)8-barrel enzymes.（βα）8桶状酶的趋异进化。
Biol Chem. 2001 Sep;382(9):1315-20. doi: 10.1515/BC.2001.163.
6
The evolution and structural anatomy of the small molecule metabolic pathways in Escherichia coli.大肠杆菌中小分子代谢途径的进化与结构解剖
J Mol Biol. 2001 Aug 24;311(4):693-708. doi: 10.1006/jmbi.2001.4912.
7
Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.酶功能的趋异进化：机制多样的超家族和功能各异的超家族。
Annu Rev Biochem. 2001;70:209-46. doi: 10.1146/annurev.biochem.70.1.209.
8
Evolution of function in protein superfamilies, from a structural perspective.从结构角度看蛋白质超家族中功能的演变。
J Mol Biol. 2001 Apr 6;307(4):1113-43. doi: 10.1006/jmbi.2001.4513.
9
Massive gene decay in the leprosy bacillus.麻风杆菌中的大规模基因衰退。
Nature. 2001 Feb 22;409(6823):1007-11. doi: 10.1038/35059006.
10
Homology among (betaalpha)(8) barrels: implications for the evolution of metabolic pathways.（β-α）8桶之间的同源性：对代谢途径进化的影响
J Mol Biol. 2000 Nov 3;303(4):627-41. doi: 10.1006/jmbi.2000.4152.
Zotero 插件