van der Zijpp Ype J T, Poot Andre A, Feijen Jan
Institute for Biomedical Technology (BMTI), Department. of Chemical Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
J Biomed Mater Res A. 2003 Apr 1;65(1):51-9. doi: 10.1002/jbm.a.10327.
Small-diameter vascular grafts rapidly fail as a result of blood coagulation and platelet deposition. Endothelial cells lining the inner side of blood vessels can provide the graft lumen with an antithrombogenic surface. One of the remaining problems is cell detachment after restoration of blood flow, because of infiltration of leukocytes that respond to an inflammatory-like activation of the endothelial cells. This endothelial activation is possibly caused by the surface characteristics of the underlying polymer. To get more insight into the effects of the polymer surface on endothelial cell activation, we seeded human umbilical vein endothelial cells (HUVECs) in various densities and subsequently grew them on tissue culture polystyrene (TCPS; hydrophilic) and polystyrene (PS; hydrophobic) surfaces. To improve cell adhesion, surfaces were coated with purified fibronectin prior to cell seeding. During proliferation, the expressions of the leukocyte adhesion molecules ICAM-1 and VCAM-1 were determined. Results indicate that ICAM-1 expression is not influenced by the character of the polymer surface, and that VCAM-1 expression is slightly higher on the TCPS surface. Expressions of both adhesion molecules are influenced by the seeding density and time of proliferation. At low seeding densities (< or = 10,000 cells/cm(2)), a relatively low percentage of nonexogenously activated cells expressed ICAM-1 during the first 3 days of proliferation compared to higher seeding densities. Although less pronounced, this was also observed for the percentage of cells expressing VCAM-1. During proliferation, the amount of ICAM-1 per endothelial cell increased, whereas the expression of VCAM-1 remained low. The absence of large differences in leukocyte adhesion molecule expression by endothelial cells grown on TCPS or PS is possibly caused by coating of the surfaces with fibronectin. It is known that surface hydrophilicity influences protein adsorption. Although this had no or little effect on leukocyte adhesion molecule expression, endothelial cell growth was affected, because proliferation was slower on the hydrophobic PS.
小口径血管移植物会因血液凝固和血小板沉积而迅速失效。血管内侧的内皮细胞可为移植物管腔提供抗血栓形成表面。剩余问题之一是恢复血流后细胞会发生脱离，这是由于白细胞浸润导致的，而白细胞浸润是对内皮细胞类似炎症激活的反应。这种内皮细胞激活可能是由底层聚合物的表面特性引起的。为了更深入了解聚合物表面对内皮细胞激活的影响，我们以不同密度接种人脐静脉内皮细胞（HUVECs），随后将它们培养在组织培养聚苯乙烯（TCPS；亲水性）和聚苯乙烯（PS；疏水性）表面上。为了改善细胞黏附，在细胞接种前用纯化的纤连蛋白包被表面。在增殖过程中，测定白细胞黏附分子ICAM - 1和VCAM - 1的表达。结果表明，ICAM - 1的表达不受聚合物表面特性的影响，而VCAM - 1在TCPS表面的表达略高。两种黏附分子的表达均受接种密度和增殖时间的影响。在低接种密度（≤10,000个细胞/cm²）下，与较高接种密度相比，在增殖的前3天，非外源性激活细胞中表达ICAM - 1的比例相对较低。虽然不太明显，但表达VCAM - 1的细胞百分比也观察到了这种情况。在增殖过程中，每个内皮细胞的ICAM - 1量增加，而VCAM - 1的表达保持较低水平。在TCPS或PS上生长的内皮细胞在白细胞黏附分子表达上没有很大差异，这可能是由于表面用纤连蛋白包被所致。已知表面亲水性会影响蛋白质吸附。虽然这对白细胞黏附分子表达没有或几乎没有影响，但内皮细胞生长受到了影响，因为在疏水性PS上增殖较慢。
