Chino Makoto, Sato Kyoko, Yamazaki Takeshi, Maitani Tamio
National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.
Shokuhin Eiseigaku Zasshi. 2002 Dec;43(6):352-5. doi: 10.3358/shokueishi.43.352.
Hokosshi extract is obtained by ethanol extraction from the seeds of hokosshi (Psoralea corylifolia), which is used as a Chinese medicine. The constituents of hokosshi extract were analyzed. The main constituent was isolated using column chromatography, and identified as bakuchiol by TLC, LC/MS and NMR. Bavachinin A was also detected. In order to prepare a marker substance for hokosshi extract, bakuchiol was isolated from seeds of hokosshi using Sep-Pak cartridges. An analytical method for hokosshi extract in foods based on detection of bakuchiol was developed. Bakuchiol was extracted from food with 60 vol% ethanol. The extract was cleaned up using a Sep-Pak plus C18 cartridge, and bakuchiol was determined by HPLC. Seasoning and juice were spiked with hokosshi extract at 500 micrograms/g and analyzed by the proposed method. The recoveries of bakuchiol were 72-99%. The detection limit for the assay was 25 micrograms/g.
补骨脂提取物是通过用乙醇从作为中药的补骨脂（Psoralea corylifolia）种子中提取而获得的。对补骨脂提取物的成分进行了分析。使用柱色谱法分离出主要成分，并通过薄层色谱法、液相色谱/质谱联用仪和核磁共振对其进行鉴定，确定为补骨脂酚。还检测到补骨脂宁A。为了制备补骨脂提取物的标志物，使用Sep - Pak柱从补骨脂种子中分离出补骨脂酚。建立了一种基于补骨脂酚检测的食品中补骨脂提取物的分析方法。用60%（体积分数）乙醇从食品中提取补骨脂酚。提取物用Sep - Pak plus C18柱净化，并用高效液相色谱法测定补骨脂酚。向调味料和果汁中添加500微克/克的补骨脂提取物，并用所提出的方法进行分析。补骨脂酚的回收率为72% - 99%。该测定方法的检测限为25微克/克。
